Claes Britt S R, Takeo Emi, Fukusaki Eiichiro, Shimma Shuichi, Heeren Ron M A
Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University.
Department of Biotechnology, Graduate School of Engineering, Osaka University.
Mass Spectrom (Tokyo). 2019;8(1):A0078. doi: 10.5702/massspectrometry.A0078. Epub 2019 Dec 27.
Mass spectrometry imaging is an imaging technology that allows the localization and identification of molecules on (biological) sample surfaces. Obtaining the localization of a compound in tissue is of great value in biological research. Yet, the identification of compounds remains a challenge. Mass spectrometry alone, even with high-mass resolution, cannot always distinguish between the subtle structural differences of isomeric compounds. This review discusses recent advances in mass spectrometry imaging of lipids, steroid hormones, amino acids and proteins that allow imaging with isomeric resolution. These improvements in detailed identification can give new insights into the local biological activity of isomers.
质谱成像技术是一种能够对（生物）样品表面的分子进行定位和识别的成像技术。在生物研究中，确定化合物在组织中的定位具有重要价值。然而，化合物的识别仍然是一项挑战。仅靠质谱，即使具有高质量分辨率，也往往无法区分同分异构体化合物的细微结构差异。本综述讨论了脂质、甾体激素、氨基酸和蛋白质质谱成像的最新进展，这些进展能够实现同分异构体分辨率成像。在详细识别方面的这些改进可以为同分异构体的局部生物活性提供新的见解。
