State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Front Cell Infect Microbiol. 2020 Feb 25;10:19. doi: 10.3389/fcimb.2020.00019. eCollection 2020.
(HPS), a member of the family Pasteurellaceae, is a common bacteria in the upper respiratory tract of pigs but under certain circumstances can cause serious systemic disease (Glasser's disease) characterized by severe infection of the upper respiratory tract, fibrinous polyserositis, polyarthritis, and meningitis. cAMP receptor protein (CRP) is among the most important global regulators, playing a vital role in adapting to environmental changes during the process of bacterial infection. In order to investigate the function of the gene in the growth characteristics of serovar 5 (HPS5) and its ability to overcome adverse environmental stresses, a mutant strain (Δcrp) was constructed and verified. In this study, we found that the gene was involved in growth rate, biofilm formation, stress tolerance, serum resistance, and iron utilization. Compared with the wild type, both the growth rate of the mutant and its resistance to osmotic pressure decreased significantly. Similar phenomena were also found in biofilm formation and iron utilization. However, the resistance to heat shock and serum complement of the mutant were enhanced. This study aimed to reveal the function in growth characteristics and stress resistance of the gene in HPS5. Whether it relates to virulence requires additional in-depth research.
（HPS）是巴斯德氏菌科的一员，是猪上呼吸道的常见细菌，但在某些情况下会引起严重的全身疾病（格拉瑟氏病），其特征是上呼吸道严重感染、纤维蛋白性多发性浆膜炎、多发性关节炎和脑膜炎。环磷酸腺苷受体蛋白（CRP）是最重要的全局调节剂之一，在细菌感染过程中适应环境变化方面发挥着重要作用。为了研究该基因在血清 5 型（HPS5）生长特性及其克服不利环境压力的能力中的功能，构建并验证了一个 基因缺失突变株（Δcrp）。在这项研究中，我们发现该基因参与了生长速度、生物膜形成、应激耐受、血清抗性和铁利用。与野生型相比，该突变株的生长速度及其对渗透压的抵抗力明显下降。在生物膜形成和铁利用方面也发现了类似的现象。然而，该突变株对热休克和血清补体的抵抗力增强。本研究旨在揭示该基因在 HPS5 生长特性和应激抗性中的功能。它是否与毒力有关还需要进一步深入研究。
