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强电解质中紫膜悬浮液的光诱导电导率变化。

Light-induced conductivity changes of purple membrane suspensions in strong electrolytes.

作者信息

Slifkin M A, Bakarudin I, Bradley S, Caplan S R

机构信息

Department of Electronic and Electrical Engineering, University of Salford, UK.

出版信息

J Bioenerg Biomembr. 1988 Oct;20(5):585-602. doi: 10.1007/BF00768921.

DOI:10.1007/BF00768921
PMID:3215903
Abstract

Measurements have been made of the modulated light-induced changes in conductivity and the associated relaxation times of bacteriorhodopsin in a variety of strong electrolytes, both unbuffered and buffered. The effects of pH and temperature variation have been studied as well as the effect of adding valinomycin. Two relaxation times can be distinguished: a fast lifetime associated with protonation-deprotonation, and a slow lifetime associated with ion binding. The ion-binding effects appear to be cation specific.

摘要

已对多种强电解质(包括未缓冲和缓冲的)中细菌视紫红质的电导率调制光诱导变化及其相关弛豫时间进行了测量。研究了pH值和温度变化的影响以及添加缬氨霉素的影响。可以区分出两种弛豫时间:与质子化 - 去质子化相关的快速寿命,以及与离子结合相关的缓慢寿命。离子结合效应似乎具有阳离子特异性。

相似文献

1
Light-induced conductivity changes of purple membrane suspensions in strong electrolytes.强电解质中紫膜悬浮液的光诱导电导率变化。
J Bioenerg Biomembr. 1988 Oct;20(5):585-602. doi: 10.1007/BF00768921.
2
Light-induced conductivity changes in purple membrane suspensions.紫膜悬浮液中光诱导的电导率变化。
Biophys Struct Mech. 1979 Aug;5(4):313-20. doi: 10.1007/BF02426665.
3
Effect of a light-induced pH gradient on purple-to-blue and purple-to-red transitions of bacteriorhodopsin.光诱导pH梯度对细菌视紫红质从紫色到蓝色以及从紫色到红色转变的影响。
Biochemistry. 1990 Jul 24;29(29):6778-88. doi: 10.1021/bi00481a005.
4
Reversible inhibition of proton release activity and the anesthetic-induced acid-base equilibrium between the 480 and 570 nm forms of bacteriorhodopsin.细菌视紫红质在480纳米和570纳米形式之间质子释放活性的可逆抑制以及麻醉诱导的酸碱平衡。
Biophys J. 1996 Feb;70(2):948-61. doi: 10.1016/S0006-3495(96)79638-8.
5
Photoelectric conversion by bacteriorhodopsin in charged synthetic membranes.带电荷合成膜中细菌视紫红质的光电转换
Biophys J. 1980 Sep;31(3):393-401. doi: 10.1016/S0006-3495(80)85067-3.
6
Light adaptation of bacteriorhodopsin correlates with dielectric spectral kinetics in purple membrane.细菌视紫红质的光适应与紫膜中的介电光谱动力学相关。
Biochem Biophys Res Commun. 2004 Mar 19;315(4):857-65. doi: 10.1016/j.bbrc.2004.01.135.
7
The effect of antibiotics on the photocycle and protoncycle of purple membrane suspensions.抗生素对紫膜悬浮液光循环和质子循环的影响。
Biochim Biophys Acta. 1979 Jan 11;545(1):15-23. doi: 10.1016/0005-2728(79)90109-9.
8
Electrooptical measurements on purple membrane containing bacteriorhodopsin mutants.对含有细菌视紫红质突变体的紫膜进行的电光测量。
Biophys J. 1996 Jan;70(1):468-72. doi: 10.1016/S0006-3495(96)79590-5.
9
Surface charge changes in purple membranes and the photoreaction cycle of bacteriorhodopsin.紫膜中的表面电荷变化与细菌视紫红质的光反应循环
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4707-11. doi: 10.1073/pnas.77.8.4707.
10
Effect of ionophoric compounds on aqueous suspensions of purple membrane.离子载体化合物对紫膜水悬浮液的影响。
FEBS Lett. 1977 Sep 15;81(2):267-70. doi: 10.1016/0014-5793(77)80532-2.

本文引用的文献

1
CHANGES IN ELECTRICAL CONDUCTANCE OF RHODOPSIN ON PHOTOLYSIS.视紫红质光解时的电导变化
J Gen Physiol. 1963 Nov;47(2):241-64. doi: 10.1085/jgp.47.2.241.
2
Salt and pH-dependent changes of the purple membrane absorption spectrum.紫膜吸收光谱的盐和pH依赖性变化。
Photochem Photobiol. 1984 Nov;40(5):641-6. doi: 10.1111/j.1751-1097.1984.tb05353.x.
3
Absolute quantum yields and proof of proton and nonproton transient release and uptake in photoexcited bacteriorhodopsin.光激发细菌视紫红质中的绝对量子产率以及质子和非质子瞬态释放与摄取的证据。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):178-80. doi: 10.1073/pnas.80.1.178.
4
Conductance changes produced by light in rod outer segments.光在视杆细胞外段产生的电导变化。
J Physiol. 1968 Oct;198(3):647-99. doi: 10.1113/jphysiol.1968.sp008631.
5
Rhodopsin-like protein from the purple membrane of Halobacterium halobium.来自嗜盐栖热菌紫膜的视紫红质样蛋白。
Nat New Biol. 1971 Sep 29;233(39):149-52. doi: 10.1038/newbio233149a0.
6
Photophosphorylation in Halobacterium halobium.嗜盐菌中的光合磷酸化作用。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1234-8. doi: 10.1073/pnas.71.4.1234.
7
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.嗜盐菌细胞膜的分离及其分成红色膜和紫色膜的分级分离。
Methods Enzymol. 1974;31:667-78. doi: 10.1016/0076-6879(74)31072-5.
8
Abrupt onset of large scale nonproton ion release in purple membranes caused by increasing pH or ionic strength.由pH值升高或离子强度增加引起的紫膜中大规模非质子离子的突然释放。
Biophys J. 1987 Jun;51(6):875-81. doi: 10.1016/S0006-3495(87)83415-X.
9
Metal ion binding sites of bacteriorhodopsin. Laser-induced lanthanide luminescence study.细菌视紫红质的金属离子结合位点。激光诱导镧系元素发光研究。
J Biol Chem. 1987 Apr 15;262(11):4947-51.
10
Structure of rhodopsin and bacteriorhodopsin.视紫红质和细菌视紫红质的结构。
Photochem Photobiol. 1987 Jun;45(6):909-14. doi: 10.1111/j.1751-1097.1987.tb07902.x.