Singh K, Korenstein R, Lebedeva H, Caplan S R
Biophys J. 1980 Sep;31(3):393-401. doi: 10.1016/S0006-3495(80)85067-3.
Photoelectroactivity of oriented purple membrane layers attached to an ion exchange film has been investigated. The action spectrum of the photocurrent followed the absorption spectrum of bacteriorhodopsin. The intactness of structure and function of bacteriorhodopsin was demonstrated by studies of absorption and photocycle kinetics. The direction of the photocurrent suggests that the extracellular surface of purple membrane is more positive. Photocurrents as high as 20 microA cm-2 were obtained in some preparations. The dependence of steady-state photocurrents on intensity of illumination and temperature was also studied. The initial rate of build-up of photocurrent depends linearly on the intensity of illumination while the off rate does not exhibit any dependence on the intensity of illumination. With rise in temperature an increase in the steady state photocurrent has been observed. This dependence was found to be linear when increase of the photocurrent due to proton translocation alone was considered.
对附着在离子交换膜上的取向紫膜层的光电活性进行了研究。光电流的作用光谱与细菌视紫红质的吸收光谱一致。通过对吸收和光循环动力学的研究，证明了细菌视紫红质结构和功能的完整性。光电流的方向表明紫膜的细胞外表面更正。在一些制剂中获得了高达20微安/平方厘米的光电流。还研究了稳态光电流对光照强度和温度的依赖性。光电流的初始建立速率与光照强度呈线性关系，而关闭速率与光照强度无关。随着温度升高，观察到稳态光电流增加。当仅考虑由于质子转运引起的光电流增加时，发现这种依赖性是线性的。
