Department of Thoracic Surgery, Affiliated Hospital of Hebei University, No. 212, Yuhuadonglu, Hebei, 071000, P.R. China.
Department of Pathology, School of Medicine, Hebei University, No. 342, Yuhuadonglu, Hebei, 071000, P.R. China.
Cell Biol Int. 2020 Jul;44(7):1447-1457. doi: 10.1002/cbin.11338. Epub 2020 Apr 20.
Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio-informatics prediction, forkhead box P4 antisense RNA 1 (FOXP4-AS1) and forkhead box P4 (FOXP4) were upregulated in esophageal carcinoma samples and were positively correlated with each other. The present study aimed to explore the function of FOXP4-AS1 and FOXP4 in ESCC cells. Function assays disclosed that knockdown of FOXP4-AS1 or FOXP4 efficiently suppressed cell proliferation and induced cell apoptosis. Moreover, FOXP4-AS1 positively regulated FOXP4 by interacting with insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) to stabilize FOXP4 messenger RNA. In addition, FOXP4-AS1 could upregulate the expression of FOXP4 by sponging miR-3184-5p. Finally, we found that Yin Yang 1 (YY1) is a transcription factor that can transcriptionally activate both FOXP4-AS1 and FOXP4 in ESCC cells. In a word, YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of ESCC cells.
食管鳞状细胞癌 (ESCC) 属于全球最常见的恶性肿瘤之一，具有较高的死亡率。长链非编码 RNA (lncRNA) 已被证明是 ESCC 进展的重要生物学参与者。基于生物信息学预测，叉头框 P4 反义 RNA 1 (FOXP4-AS1) 和叉头框 P4 (FOXP4) 在食管癌样本中上调，并且彼此呈正相关。本研究旨在探讨 FOXP4-AS1 和 FOXP4 在 ESCC 细胞中的功能。功能测定表明，敲低 FOXP4-AS1 或 FOXP4 可有效抑制细胞增殖并诱导细胞凋亡。此外，FOXP4-AS1 通过与胰岛素样生长因子 2 mRNA 结合蛋白 2 (IGF2BP2) 相互作用正向调节 FOXP4，以稳定 FOXP4 信使 RNA。此外，FOXP4-AS1 可以通过海绵 miR-3184-5p 上调 FOXP4 的表达。最后，我们发现 Yin Yang 1 (YY1) 是一种转录因子，可在 ESCC 细胞中转录激活 FOXP4-AS1 和 FOXP4。总之，YY1 诱导的 FOXP4-AS1 和 FOXP4 上调促进了 ESCC 细胞的增殖。
