采用高效液相色谱-荧光检测法测定β-咔啉类化合物。

Measurement of beta-carbolines by high-performance liquid chromatography with fluorescence detection.

作者信息

Bosin T R, Faull K F

机构信息

Pharmacology Section, Indiana University School of Medicine, Bloomington 47405.

出版信息

J Chromatogr. 1988 Jul 15;428(2):229-36. doi: 10.1016/s0378-4347(00)83913-4.

DOI:10.1016/s0378-4347(00)83913-4

PMID:3215926

Abstract

A method using high-performance liquid chromatography with fluorescence detection was developed for the determination of beta-carboline compounds norharman, harman, norharmol, and harmol in lung. Aqueous derivatization with acetic anhydride was used to facilitate the isolation and separation of the phenolic compounds and to reduce the fluorescence background of the biological samples. Harman was identified and quantitated in rat lung (1.88 +/- 0.55 ng/g) using this method and its identity confirmed by means of gas chromatography-negative-ion chemical ionization mass spectrometry.

摘要

开发了一种采用带荧光检测的高效液相色谱法测定肺中β-咔啉化合物去甲哈尔满、哈尔满、去甲哈尔醇和哈尔醇的方法。使用乙酸酐进行水相衍生化，以促进酚类化合物的分离，并降低生物样品的荧光背景。采用该方法在大鼠肺中鉴定并定量了哈尔满（1.88±0.55 ng/g），并通过气相色谱-负离子化学电离质谱法确认了其身份。