Silva-Sousa Alice Corrêa, Mazzi-Chaves Jardel Francisco, Freitas Jessica Vavassori, Salles Alessandro Guimarães, Segato Raquel Assed Bezerra da Silva, Silva Léa Assed Bezerra da, Antunes Lívia Azeredo Alves, Antunes Leonardo Santos, Baratto-Filho Flares, Sousa-Neto Manoel Damião, Küchler Erika Calvano
School of Dentistry,UNAERP - Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brazil.
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, USP- Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2020 Jan-Feb;31(1):19-24. doi: 10.1590/0103-644020200.
This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the "healed" group and 73 in the "PAP" group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.
本研究评估了编码雌激素受体1（ESR1）和2（ESR2）、维生素D受体（VDR）的基因多态性以及微小RNA17（其与ESR1和VDR结合）与根管治疗后持续性根尖周炎（PAP）之间的关联。我们纳入了162例至少在一年前完成根管治疗且在根管治疗开始时患有根尖周炎的患者。进行了临床和影像学检查，以评估PAP或健康根尖周组织（愈合）的存在情况。收集唾液样本作为基因组DNA。通过实时聚合酶链反应对ESR1（rs2234693和rs9340799）、ESR2（rs1256049和rs4986938）、VDR（rs739837和rs2228570）和微小RNA17（rs4284505）进行基因分型。采用卡方检验分析基因型和等位基因频率的分布。还进行了单倍型分析。“愈合”组纳入89例患者，“PAP”组纳入73例患者。在所研究的等位基因和基因型多态性与PAP之间未发现关联（p>0.05）。单倍型分析也未显示出关联（p>0.05）。总之，ESR1、ESR2、VDR和微小RNA17的基因多态性与PAP无关。
