Küchler Erika Calvano, Gerlach Raquel Fernanda, Cunha Arthur S, Ramazzotto Lucas A, Spada Paula Porto, Nelson-Filho Paulo, Ramos Junia, Pecharki Giovana Daniela, Barbosa Fernando, Vieira Alexandre Rezende, Trevilatto Paula Cristina, Brancher João Armando
Department of Pediatric Dentistry, Ribeirão Preto Dental School, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dental School, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2020 Sep-Oct;31(5):466-470. doi: 10.1590/0103-6440202002934.
Homeostasis between salivary calcium and phosphorus is important for maintaining oral health. The aim of this study was to evaluate if polymorphisms in ESR1 (Estrogen Receptor Alpha), ESR2 (Estrogen Receptor Beta) and miRNA17 (microRNA17) are associated with calcium and phosphorus levels in saliva. Saliva from 276 12-year-old children were collected by masticatory stimulation and calcium and phosphorus levels were determined by Mass Spectrometry. Genomic DNA was extracted from remaining saliva and genetic polymorphisms in ESR1 (rs12154178, rs1884051, rs9340799 and rs2234693), in ESR2 (rs4986938 and rs1256049) and in miRNA17 (rs4284505) were genotyped using TaqMan chemistry and a real-time PCR equipment. Statistical differences in genotype and allele distributions between 'low' and 'high' calcium and phosphorus levels were determined using chi-square or Fisher´s exact tests. The analysis was also adjusted by sex (alpha of 5%). ESR1 rs9340799 had the less common genotype associated with higher calcium levels (p=0.03). The less common allele of ESR1 rs1884051 was associated with lower phosphorus levels (p=0.005) and there was an excess of heterozygotes for miRNA17 rs4284505 among individuals with lower calcium levels (p=0.002), both adjusted by sex. This study provides evidence that genetic polymorphisms in ESR1 and miRNA17 are involved in determining salivary calcium and phosphorus levels.
唾液中钙和磷的稳态对于维持口腔健康很重要。本研究的目的是评估雌激素受体α（ESR1）、雌激素受体β（ESR2）和微小RNA17（miRNA17）的多态性是否与唾液中的钙和磷水平相关。通过咀嚼刺激收集了276名12岁儿童的唾液，并通过质谱法测定了钙和磷水平。从剩余的唾液中提取基因组DNA，并使用TaqMan化学方法和实时PCR设备对ESR1（rs12154178、rs1884051、rs9340799和rs2234693）、ESR2（rs4986938和rs1256049）以及miRNA17（rs4284505）中的基因多态性进行基因分型。使用卡方检验或Fisher精确检验确定“低”和“高”钙磷水平之间基因型和等位基因分布的统计学差异。分析也按性别进行了校正（α = 5%）。ESR1 rs9340799具有与较高钙水平相关的较少见基因型（p = 0.03）。ESR1 rs1884051的较少见等位基因与较低磷水平相关（p = 0.005），并且在钙水平较低的个体中，miRNA17 rs4284505的杂合子过多（p = 0.002），两者均按性别进行了校正。本研究提供了证据表明ESR1和miRNA17中的基因多态性参与了唾液钙和磷水平的决定。
