Advanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Ocul Immunol Inflamm. 2021 Nov 17;29(7-8):1445-1451. doi: 10.1080/09273948.2020.1734212. Epub 2020 Mar 11.
: To evaluate the role of angiogenic growth factors in the pathogenesis of intraocular tuberculosis.: Retinal Pigment Epithelium (RPE) cells were infected with varying dilution of (MTB), ranging from several thousand to a few MTB bacilli to replicate paucibacillary conditions. Angiogenesis growth factors were evaluated using multiplex fluorescent bead based flow cytometry in the culture supernatant of RPE cells infected with MTB, vitreous fluids and tear samples of uveitis patients visiting retina clinic.: Vascular endothelial growth factor (VEGF) levels were elevated and fibroblast growth factors (FGFs) were down regulated in RPE-infected MTB cells. Similar pattern of VEGF and FGF was observed in the vitreous of IOTB patients. However, no changes were observed in tear samples.: MTB exploits the angiogenesis growth factors for pathogenesis by decreasing FGF with concomitant surge of VEGF in MTB infected RPE as well in the vitreous of IOTB patients.
: 评价血管生成生长因子在眼内结核发病机制中的作用。: 用不同稀释度（MTB）感染视网膜色素上皮（RPE）细胞，范围从几千到几个 MTB 杆菌，以复制少量杆菌条件。用基于多重荧光珠的流式细胞术评估 MTB 感染的 RPE 细胞、葡萄膜炎患者的玻璃体和泪液样本中的血管生成生长因子。: 在 RPE 感染 MTB 细胞、眼内结核患者的玻璃体中，血管内皮生长因子（VEGF）水平升高，成纤维细胞生长因子（FGFs）下调。在 IOTB 患者的玻璃体中也观察到类似的 VEGF 和 FGF 模式。然而，在泪液样本中未观察到变化。: MTB 通过在感染 MTB 的 RPE 中减少 FGF 并伴随 VEGF 的激增，以及在 IOTB 患者的玻璃体中利用血管生成生长因子进行发病。
