Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Ya'acob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Department of Emergency Medicine, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia Medical Centre, Jalan Ya'acob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
PLoS One. 2020 Mar 11;15(3):e0230285. doi: 10.1371/journal.pone.0230285. eCollection 2020.
The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS.
本研究旨在确定在患有细菌性败血症综合征（BSS）的患者中，分泌型磷脂酶 A2 组 IIA（sPLA2-IIA）与类二十烷酸代谢物之间的关联。从确诊为细菌性败血症（BS；N=45）、严重细菌性败血症/败血症性休克（BSS/SS；N=35）和健康受试者（N=45）的血清中定量测定 sPLA2-IIA、类二十烷酸前列腺素（PG）E2、PGD 合酶的水平。从细胞裂解物中分析环加氧酶（COX）-1 和 COX-2 活性。与健康受试者相比，BS 和 BSS/SS 患者的血清 sPLA2-IIA、PGE2 和 PGDS 水平显着升高（p<0.05）。与健康受试者相比，BS 患者的 COX-2 活性显着升高（p<0.05），但 COX-1 活性没有升高。二元逻辑回归分析表明，sPLA2-IIA 和 PGE2 是预测 BSS 严重程度的独立因素。总之，高水平的 sPLA2-IIA 与 BSS 患者的类二十烷酸代谢有关。
