Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea.
Department of Life Science, Kyonggi University, Suwon 16227, Korea.
Am J Chin Med. 2020;48(2):429-444. doi: 10.1142/S0192415X20500226. Epub 2020 Mar 11.
Pharmacological activities of some Leguminosae family members were reported. Pharmacological activities of , a Leguminosae family member have never been explored. Therefore, this study investigated anti-inflammatory effects of an methanol extract (Al-ME). In this study, anti-inflammatory effects of Al-ME were investigated in LPS-stimulated RAW264.7 cells and HCl/EtOH-induced gastritis mice by MTT assay, nitric oxide (NO) production assay, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), luciferase reporter assay, and Western blotting. High-performance liquid chromatography (HPLC) analysis identified ethnopharmacological compounds in Al-ME. Al-ME inhibited NO production without cytotoxicity in peritoneal macrophages and RAW264.7 cells stimulated with LPS or Pam3CSK4. Al-ME downregulated mRNA expression of inflammatory genes (inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2)) and pro-inflammatory cytokines (tumor necrosis factor- (TNF-), interleukin-1 (IL-1), and IL-6). Al-ME exerted anti-inflammatory activity in LPS-stimulated RAW264.7 cells by inhibiting nuclear factor-kappa B (NF-B) signaling pathway. HPLC analysis identified quercetin, luteolin, and kaempferol as major anti-inflammatory components in Al-ME. Al-ME ameliorated HCl/EtOH-induced gastritis symptoms in mice by suppressing iNOS and IL-6 mRNA expressions and IB phosphorylation. Therefore, these results suggest that Al-ME exhibited anti-inflammatory activity by targeting NF-B signaling pathway, implying that Al-ME could be potent anti-inflammatory medications to prevent and treat inflammatory diseases.
一些豆科家族成员的药理活性已被报道。豆科家族成员的药理活性从未被探索过。因此，本研究调查了一种甲醇提取物（Al-ME）的抗炎作用。在这项研究中，通过 MTT 测定法、一氧化氮（NO）产生测定法、半定量逆转录-聚合酶链反应（RT-PCR）、荧光素酶报告测定法和 Western blot 分析，研究了 Al-ME 在 LPS 刺激的 RAW264.7 细胞和 HCl/EtOH 诱导的胃炎小鼠中的抗炎作用。高效液相色谱（HPLC）分析鉴定了 Al-ME 中的民族药理学化合物。Al-ME 抑制了 LPS 或 Pam3CSK4 刺激的腹腔巨噬细胞和 RAW264.7 细胞中 NO 的产生，而没有细胞毒性。Al-ME 下调了炎症基因（诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2））和促炎细胞因子（肿瘤坏死因子-（TNF-），白细胞介素-1（IL-1）和 IL-6）的 mRNA 表达。Al-ME 通过抑制核因子-kappa B（NF-B）信号通路在 LPS 刺激的 RAW264.7 细胞中发挥抗炎作用。HPLC 分析鉴定出 Al-ME 中的主要抗炎成分是槲皮素、木犀草素和山奈酚。Al-ME 通过抑制 iNOS 和 IL-6 mRNA 表达和 IB 磷酸化，改善了 HCl/EtOH 诱导的胃炎小鼠的症状。因此，这些结果表明，Al-ME 通过靶向 NF-B 信号通路发挥抗炎作用，表明 Al-ME 可能是预防和治疗炎症性疾病的有效抗炎药物。
