Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Anatomical Sciences, Faculty of Medicine; Torabinejad Dental Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran.
Curr Pharm Des. 2020;26(26):3147-3160. doi: 10.2174/1381612826666200311124732.
Polyhedral oligomeric silsesquioxane (POSS) is a monomer with silicon structure and an internal nanometric cage.
The purpose of this study was to provide an injectable hydrogel that could be easily located in open or closed bone fractures and injuries, and also to reduce the possible risks of infections caused by bone graft either as an allograft or an autograft.
Various formulations of temperature sensitive hydrogels containing hydroxyapatite, Gelrite, POSS and platelets rich plasma (PRP), such as the co-gelling agent and cell growth enhancer, were prepared. The hydrogels were characterized for their injectability, gelation time, phase transition temperature and viscosity. Other physical properties of the optimized formulation including compressive stress, compressive strain and Young's modulus as mechanical properties, as well as storage and loss modulus, swelling ratio, biodegradation behavior and cell toxicity as rheometrical parameters were studied on human osteoblast MG-63 cells. Alizarin red tests were conducted to study the qualitative and quantitative osteogenic capability of the designed scaffold, and the cell adhesion to the scaffold was visualized by scanning electron microscopy.
The results demonstrated that the hydrogel scaffold mechanical force and injectability were 3.34±0.44 Mpa and 12.57 N, respectively. Moreover, the scaffold showed higher calcium granules production in alizarin red staining compared to the control group. The proliferation of the cells in G4.5H1P0.03PRP10 formulation was significantly higher than in other formulations (p<0.05).
The optimized Gelrite/Hydroxyapatite/POSS/PRP hydrogel scaffold has useful impacts on osteoblasts activity, and may be beneficial for local drug delivery in complications including a break or bone loss.
多面体低聚倍半硅氧烷（POSS）是一种具有硅结构和内部纳米笼的单体。
本研究旨在提供一种可注射水凝胶，可轻松定位在开放性或闭合性骨折和损伤部位，同时降低因同种异体或自体移植物而导致感染的可能性。
制备了含有羟基磷灰石、Gelrite、POSS 和富含血小板的血浆（PRP）的各种温敏水凝胶配方，如共凝胶剂和细胞生长增强剂。对水凝胶的可注射性、凝胶时间、相转变温度和粘度进行了表征。对优化配方的其他物理性质，包括作为力学性能的压缩应力、压缩应变和杨氏模量，以及作为流变学参数的储能模量和损耗模量、溶胀比、生物降解行为和细胞毒性进行了研究。通过茜素红试验研究了设计支架的定性和定量成骨能力，并通过扫描电子显微镜观察了细胞对支架的黏附情况。
结果表明，水凝胶支架的力学强度和可注射性分别为 3.34±0.44 MPa 和 12.57 N。此外，与对照组相比，支架在茜素红染色中显示出更高的钙颗粒生成。在 G4.5H1P0.03PRP10 配方中，细胞的增殖明显高于其他配方（p<0.05）。
优化的 Gelrite/羟基磷灰石/POSS/PRP 水凝胶支架对成骨细胞活性有有益的影响，可能有利于局部药物输送在包括骨折或骨丢失在内的并发症中。
