Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmaceutics, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
Pharm Dev Technol. 2021 Feb;26(2):220-232. doi: 10.1080/10837450.2020.1858318. Epub 2020 Dec 27.
Novel osteoinductive scaffolds fabricated using the benefits of tissue engineering techniques accompanied by utilizing drugs can accelerate bone regeneration. The purpose of this study was to load salmon calcitonin (sCT) in octamaleimic acid-silsesquioxane (OMA-POSS) nanoparticles and enrich the hydrogel scaffold based on hydroxyapatite, Gelrite® and platelet-rich plasma (PRP) for use in bone tissue engineering. The loading efficiency, release percentage, particle size and zeta potential of the nanoparticles were evaluated. The proliferation of seeded MG-63 osteoblast cells on the designed scaffold, its cytotoxicity and osteo-conductivity were studied by alkaline phosphatase measurement and Alizarin red staining. The expression of cellular osteogenic markers such as collagen 1 (COL1A1), osteocalcin (BGLAP) and osteopontin (SPP1) was examined using reverse transcription polymerase chain reaction. The results revealed that the particle size of the nanoparticles varied between 94.2 and 199.2 nm and their negative surface charge increased after drug conjugation. The osteoblast cell proliferation and calcium granule production in the optimum formulation were significantly higher in comparison with the control group ( < 0.05). Osteogenic markers increased significantly after a specific number of days of cell culture compared to the control group ( < 0.05). The results also showed the potential of the designed scaffold in bone tissue engineering.
利用组织工程技术的优势制造新型骨诱导支架，并结合药物使用，可以加速骨再生。本研究的目的是将鲑鱼降钙素（sCT）载入八异氰酸根合硅氧烷- POSS（OMA-POSS）纳米粒子中，并在基于羟基磷灰石、Gelrite®和富含血小板的血浆（PRP）的水凝胶支架中进行富集，用于骨组织工程。评估了纳米粒子的载药效率、释放百分比、粒径和zeta 电位。通过碱性磷酸酶测量和茜素红染色研究了设计的支架上种子 MG-63 成骨细胞的增殖、细胞毒性和骨诱导性。通过逆转录聚合酶链反应检测细胞成骨标志物，如胶原 1（COL1A1）、骨钙素（BGLAP）和骨桥蛋白（SPP1）的表达。结果表明，纳米粒子的粒径在 94.2 和 199.2nm 之间变化，药物偶联后其表面负电荷增加。与对照组相比，最佳配方中成骨细胞的增殖和钙颗粒生成显著增加（<0.05）。与对照组相比，细胞培养特定天数后成骨标志物显著增加（<0.05）。结果还表明，设计的支架在骨组织工程中有应用潜力。
