Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Department of Orthopedic Surgery, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.
J Orthop Surg Res. 2020 Mar 11;15(1):102. doi: 10.1186/s13018-020-01593-x.
Large segmental bone defects are still one of the challenges for orthopaedic surgeons. Although 3D-printed porous titanium is a potential bone substitute material because of its porous structure simulating natural bone, the titanium surface has low bioactivity, integrates with bone tissue through the simple mechanical interlock. The study aims to investigate the capability and osteogenesis of 3D-printed porous titanium (3D PPT)-coated polydopamine (PDA) for repairing bone defects.
Fifteen 6-month New Zealand white rabbits were implanted with PDA-3D PPT to repair 6 mm × 10 mm defects on the femoral condyle compared with the group of 3D PPT and comparing with the blank group. After 6 weeks and 12 weeks, micro-CT and histological examination were performed to observe bone growth.
All the PDA-3D PPT group, the 3D PPT group and the blank group recovered in good condition. The images showed that the boundaries between the implant area and the surrounding area were obscure in the three groups. The results of micro-CT demonstrated that at 6 weeks and 12 weeks, the bone volume (BV) values of PDA-3D PPT implants group were significantly higher than those of the 3D PPT implants group and blank group (P < 0.05), the BV/tissue volume (TV) and the trabecular number (Tb.N) of PDA-3D PPT implants were significantly higher than those of the 3D PPT group and blank group (P < 0.05). The results of un-decalcified bone slicing showed that ore new bone appeared to form around the PDA-3D PPT than that of 3D PPT and blank group. The bone-implant contact (BIC) of PDA-3D PPT was better (P < 0.05) than that of 3D PPT group.
PDA-3D PPT could improve the bioactivity and promote the growth and healing of bone tissue and can be a promising repairing material.
大段骨缺损仍然是矫形外科医生面临的挑战之一。尽管 3D 打印多孔钛因其模拟天然骨的多孔结构而成为一种有潜力的骨替代材料，但钛表面的生物活性低，通过简单的机械联锁与骨组织结合。本研究旨在探讨 3D 打印多孔钛（3D PPT）-聚多巴胺（PDA）涂层修复骨缺损的能力和成骨作用。
将 PDA-3D PPT 植入 15 只 6 月龄新西兰白兔股骨髁 6mm×10mm 缺损处，与 3D PPT 组和空白组进行比较。6 周和 12 周后，进行微 CT 和组织学检查观察骨生长情况。
所有 PDA-3D PPT 组、3D PPT 组和空白组均恢复良好。图像显示，三组植入区与周围区边界模糊。微 CT 结果显示，6 周和 12 周时，PDA-3D PPT 植入组的骨体积（BV）值明显高于 3D PPT 植入组和空白组（P<0.05），BV/组织体积（TV）和小梁数（Tb.N）明显高于 3D PPT 植入组和空白组（P<0.05）。脱钙骨切片结果显示，PDA-3D PPT 周围新骨形成多于 3D PPT 和空白组。PDA-3D PPT 的骨-植入物接触（BIC）优于 3D PPT 组（P<0.05）。
PDA-3D PPT 能提高生物活性，促进骨组织的生长和愈合，是一种有前途的修复材料。
