Wimbish Robert T, DeLuca Jennifer G
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, United States.
Front Cell Dev Biol. 2020 Feb 26;8:43. doi: 10.3389/fcell.2020.00043. eCollection 2020.
Successful mitotic cell division is critically dependent on the formation of correct attachments between chromosomes and spindle microtubules. Microtubule attachments are mediated by kinetochores, which are large proteinaceous structures assembled on centromeric chromatin of mitotic chromosomes. These attachments must be sufficiently stable to transduce force; however, the strength of these attachments are also tightly regulated to ensure timely, error-free progression through mitosis. The highly conserved, kinetochore-associated NDC80 complex is a core component of the kinetochore-microtubule attachment machinery in eukaryotic cells. A small, disordered region within the Hec1 subunit of the NDC80 complex - the N-terminal "tail" domain - has been actively investigated during the last decade due to its roles in generating and regulating kinetochore-microtubule attachments. In this review, we discuss the role of the NDC80 complex, and specifically the Hec1 tail domain, at the kinetochore-microtubule interface, and how recent studies provide a more unified view of Hec1 tail domain function.
有丝分裂细胞的成功分裂严重依赖于染色体与纺锤体微管之间形成正确的附着。微管附着由动粒介导，动粒是在有丝分裂染色体着丝粒染色质上组装的大型蛋白质结构。这些附着必须足够稳定以传递力；然而，这些附着的强度也受到严格调控，以确保有丝分裂过程能够及时、无误地进行。高度保守的、与动粒相关的NDC80复合体是真核细胞中动粒 - 微管附着机制的核心组成部分。在过去十年中，由于NDC80复合体的Hec1亚基内一个小的无序区域——N端“尾巴”结构域——在产生和调节动粒 - 微管附着中所起的作用，它一直受到积极研究。在这篇综述中，我们讨论了NDC80复合体，特别是Hec1尾巴结构域在动粒 - 微管界面的作用，以及最近的研究如何为Hec1尾巴结构域的功能提供了更统一的观点。
