Pu Feifei, Feng Jing, Niu Fei, Xia Ping
Department of Orthopedics, Wuhan No.1 Hospital, Wuhan Integrated TCM & Western Medicine Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.215, Zhongshan Road, Wuhan, Hubei 430022, P.R. China.
School of Medicine, Jianghan University, Wuhan, Hubei 430000, P.R. China.
Open Med (Wars). 2020 Feb 28;15:114-118. doi: 10.1515/med-2020-0017. eCollection 2020.
To explore the diagnostic value of recombinant heparin-binding hemagglutinin adhesin (HBHA) protein antigen in spinal tuberculosis.
Forty patients with spinal tuberculosis were included in the experimental group and 40 healthy people were included in the control group. Serum IgG antibody expression level was detected with recombinant HBHA protein as the antigen, using enzyme-linked immunosorbent assay (ELISA) detection.
Patients with spinal tuberculosis and healthy volunteers were included in this study. A total of 40 eligible patients with spinal tuberculosis were included (24 males and 16 females, aged 18-72 years, with an average age of 41.24 ± 15.74 years). Forty healthy people were included (21 males and 19 females, aged 18-70 years, with an average age of 41.33 ± 12.36 years). On comparing the groups, no significant difference was found in the general data (P >0.05). IgG antibody level in the experimental group was higher than that in the control group, and the difference was significant (P < 0.00001).
Detection of serum HBHA protein antibody is of great value in the auxiliary diagnosis of spinal tuberculosis, and high HBHA expression can be used as an indicator for diagnosis of spinal tuberculosis.
探讨重组肝素结合血凝素黏附素（HBHA）蛋白抗原在脊柱结核中的诊断价值。
实验组纳入40例脊柱结核患者，对照组纳入40名健康人。以重组HBHA蛋白为抗原，采用酶联免疫吸附测定（ELISA）检测血清IgG抗体表达水平。
本研究纳入脊柱结核患者及健康志愿者。共纳入40例符合条件的脊柱结核患者（男24例，女16例，年龄18 - 72岁，平均年龄41.24±15.74岁）。纳入40名健康人（男21例，女19例，年龄18 - 70岁，平均年龄41.33±12.36岁）。比较两组，一般资料差异无统计学意义（P>0.05）。实验组IgG抗体水平高于对照组，差异有统计学意义（P<0.00001）。
血清HBHA蛋白抗体检测对脊柱结核的辅助诊断具有重要价值，HBHA高表达可作为脊柱结核的诊断指标。
