Rajendra K C, Zosky Graeme R, Shukla Shakti D, O'Toole Ronan F
College of Health and Medicine, University of Tasmania, Hobart, Tasmania, Australia.
School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales, Australia.
Biol Methods Protoc. 2018 Oct 4;3(1):bpy010. doi: 10.1093/biomethods/bpy010. eCollection 2018.
Nearly half of the world's population uses biomass fuel for the purposes of cooking and heating. Smoke derived from biomass increases the risk of the development of lung diseases, including pneumonia, chronic obstructive pulmonary disease, airway tract infections, and lung cancer. Despite the evidence linking biomass smoke exposure to pulmonary disease, only a small number of experimental studies have been conducted on the impact of biomass smoke on airway epithelial cells. This is in part due to the lack of a standard and easily accessible procedure for the preparation of biomass smoke. Here, we describe a cost-effective and reproducible method for the generation of different smoke extracts, in particular, cow dung smoke extract (CDSE) and wood smoke extract (WSE) for use in a range of biological applications. We examined the effect of the biomass smoke extracts on human bronchial epithelial cell expression of a known responder to cigarette smoke exposure (CSE), the platelet-activating factor receptor (PAFR). Similar to the treatment with CSE, we observed a dose-dependent increase in PAFR expression on human airway epithelial cells that were exposed to CDSE and WSE. This method provides biomass smoke in a re-usable form for cell and molecular bioscience studies on the pathogenesis of chronic lung disease.
世界上近一半的人口使用生物质燃料做饭和取暖。生物质产生的烟雾会增加患肺部疾病的风险，包括肺炎、慢性阻塞性肺疾病、呼吸道感染和肺癌。尽管有证据表明接触生物质烟雾与肺部疾病有关，但关于生物质烟雾对气道上皮细胞影响的实验研究却很少。部分原因是缺乏制备生物质烟雾的标准且易于操作的程序。在此，我们描述了一种经济高效且可重复的方法来生成不同的烟雾提取物，特别是用于一系列生物学应用的牛粪烟雾提取物（CDSE）和木烟雾提取物（WSE）。我们研究了生物质烟雾提取物对人支气管上皮细胞中已知的香烟烟雾暴露（CSE）反应蛋白——血小板活化因子受体（PAFR）表达的影响。与用CSE处理相似，我们观察到暴露于CDSE和WSE的人气道上皮细胞上PAFR表达呈剂量依赖性增加。这种方法为慢性肺病发病机制的细胞和分子生物科学研究提供了可重复使用的生物质烟雾。
