Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai, 536007, Guangxi, China.
Ecotoxicology. 2020 Sep;29(7):932-940. doi: 10.1007/s10646-020-02185-5. Epub 2020 Mar 11.
Seagrass meadows are among the four most productive marine natural ecosystems in the world. Zostera japonica (Z. japonica) is the most widely distributed species of seagrass in China. Nucleotide exchange factors (NEFs) promote the release of ADP during heat stress, accelerating the rate-limiting step of Heat shock protein 70 (Hsp70). Although NEFs play an important role in abiotic stress tolerance of plants, NEFs in seagrass have not been studied. In this study, we cloned Fes1 from Z. japonica (ZjFes1) by rapid amplification of the cDNA ends using RACE, and full length ZjFes1 was 1171 bp. It contained an 81 bp 5'-terminal untranslated region (UTR), 109 bp 3'-UTR and 981 bp open reading frame (ORF). The ORF (ZjFes1) was predicted to encode a polypeptide of 326 amino acids with theoretical molecular weight (MW) of 36.10 kDa and pI of 5.22. ZjFes1 shared 89% amino acid identity with Fes1 from Zostera marina (Z. marina). The transcriptional levels of ZjFes1 increased significantly 1 h after heat treatment. ZjFes1 was localized to the cytoplasm. Taken together, we found that ZjFes1 was a stress-inducible gene that may be involved in heat stress response. This study lays the foundation for further studies on the role of ZjFes1 in heat resistance.
海草床是世界上四大生产力最高的海洋自然生态系统之一。 日本鳗草（Zostera japonica）是中国分布最广的海草物种。核苷酸交换因子（NEFs）在热应激期间促进 ADP 的释放，从而加速热休克蛋白 70（Hsp70）的限速步骤。尽管 NEFs 在植物的非生物胁迫耐受中发挥着重要作用，但海草中的 NEFs 尚未得到研究。在这项研究中，我们通过使用 RACE 的 cDNA 末端快速扩增克隆了来自日本鳗草（ZjFes1）的 Fes1，并获得了全长 ZjFes1，其长度为 1171bp。它包含 81bp 的 5'-非翻译区（UTR），109bp 的 3'-UTR 和 981bp 的开放阅读框（ORF）。ORF（ZjFes1）预测编码一个由 326 个氨基酸组成的多肽，理论分子量（MW）为 36.10kDa，等电点（pI）为 5.22。ZjFes1 与来自 Zostera marina（Z. marina）的 Fes1 具有 89%的氨基酸同一性。热处理 1 小时后，ZjFes1 的转录水平显著增加。ZjFes1 定位于细胞质。总之，我们发现 ZjFes1 是一种应激诱导基因，可能参与热应激反应。这项研究为进一步研究 ZjFes1 在耐热性中的作用奠定了基础。
