Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Core Facilities - Clinical Proteomics and Metabolomics, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
FASEB J. 2020 May;34(5):6322-6334. doi: 10.1096/fj.201900738RR. Epub 2020 Mar 12.
Maternal nutrition during pregnancy influences offspring health. Dietary supplementation of pregnant women with (n-3) long-chain polyunsaturated fatty acids (PUFA) was shown to exert beneficial effects on offspring, through yet unknown mechanisms. Here, we conducted a dietary intervention study on a cohort of 10 women diagnosed with threatened preterm labor with a nutritional integration with eicosapentaenoic and docosahexaenoic acids. Microvesicles (MV) isolated form arterial cord blood of the treated cohort offspring and also of a randomized selection of 10 untreated preterm and 12 term newborns, were characterized by dynamic light scattering and analyzed by proteomic and statistical analysis. Glutathione synthetase was the protein bearing the highest discrimination ability between cohorts. ELISA assay showed that glutathione synthetase was more abundant in cord blood from untreated preterm compared to the other conditions. Assay of free SH-groups showed that serum of preterm subjects was oxidized. Data suggest that preterm suffer from oxidative stress, which was lower in the treated cohort. This study confirms that MV are a representative sample of the individual status and the efficacy of dietary intervention with PUFA in human pregnancy in terms of lowered inflammatory status, increased gestational age and weight at birth.
孕期母体营养会影响后代健康。给孕妇补充（n-3）长链多不饱和脂肪酸（PUFA）已被证明通过未知机制对子代产生有益影响。在这里，我们对一组被诊断为先兆早产的 10 名妇女进行了饮食干预研究，她们接受了 EPA 和 DHA 的营养整合治疗。从治疗组后代的动脉脐带血和随机选择的 10 名未治疗的早产和 12 名足月新生儿中分离出的微囊泡（MV），通过动态光散射进行了特征描述，并通过蛋白质组学和统计分析进行了分析。谷胱甘肽合酶是区分队列的蛋白中具有最高区分能力的蛋白。ELISA 检测显示，未治疗的早产儿脐带血中谷胱甘肽合酶的含量高于其他情况。自由 SH 基团检测显示，早产儿的血清发生了氧化。数据表明，早产儿患有氧化应激，而治疗组的氧化应激较低。这项研究证实，MV 是个体状态的代表性样本，并且在人类妊娠中用 PUFA 进行饮食干预具有降低炎症状态、增加胎龄和出生体重的功效。
