Selim Ahmed Magdy, Senapati Santosh Kumar, Das Manoranjan, Mishra Chinmoy, Patra Ramesh Chandra, Panda Susen Kumar
Internal Medicine, Infectious and Fish diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Clinical Medicine, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, India.
Anim Biotechnol. 2021 Dec;32(6):663-670. doi: 10.1080/10495398.2020.1738450. Epub 2020 Mar 12.
The objective of the present study was to investigate the epidemiological and haematological parameters with simultaneous molecular detection of infection of crossbred jersey (CBJ) cattle. Haematological values like mean hemoglobin (Hb) (7.31 ± 2.3 g/dl), packed cell volume (PCV) (21.69 ± 6.11%), red blood cells count (RBCs) (4.40 ± 1.6 M/μl), white blood cells count (WBCs) (6.93 ± 3.06 103/µl) and mean corpuscular hemoglobin concentration (MCHC) (33.56 ± 3.51 g/dl) were decreased significantly ( < 0.05), whereas mean corpuscular volume (MCV) (51.06 ± 6.14fl) and eosinophil count (0.39 ± 0.44 103/µl) were significantly ( < 0.05) increased in cattle infected with . Analysis of major piroplasm surface protein (MPSP) of 110 blood samples randomly collected from cattle from seven districts by PCR indicated that an average of 70% of cattle was positive for infection. In particular, Puri and Khorda districts were identified as relatively high-risk areas for infection, with infection rates of 76.66% and 72.4%, respectively. The phylogenetic analysis of isolated MPSP gene (MN334767) classified it into type 5. Earlier Indian isolates were classified into three types .type 1, type 3 and type 7 and this is the first time to detect type 5 in Odisha, India.
本研究的目的是调查杂交泽西牛（CBJ）感染的流行病学和血液学参数，并同时进行分子检测。血液学指标如平均血红蛋白（Hb）（7.31±2.3g/dl）、红细胞压积（PCV）（21.69±6.11%）、红细胞计数（RBCs）（4.40±1.6M/μl）、白细胞计数（WBCs）（6.93±3.06×10³/μl）和平均红细胞血红蛋白浓度（MCHC）（33.56±3.51g/dl）显著降低（P<0.05），而感染牛的平均红细胞体积（MCV）（51.06±6.14fl）和嗜酸性粒细胞计数（0.39±0.44×10³/μl）显著升高（P<0.05）。通过PCR对从七个地区的牛中随机采集的110份血液样本的主要梨形虫表面蛋白（MPSP）进行分析，结果表明平均70%的牛感染呈阳性。特别是，普里和科尔达地区被确定为感染的相对高风险地区，感染率分别为76.66%和72.4%。对分离的梨形虫MPSP基因（MN334767）进行系统发育分析，将其分类为5型。早期印度分离株分为三种类型，即1型、3型和7型，这是首次在印度奥里萨邦检测到5型。
