Zelenikhin P V, Ead Mohamed I S, Nadyrova A I, Sirotkina A A, Ulyanova V V, Mironova N L, Mitkevich V A, Makarov A A, Zenkova M A, Ilinskaya O N
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008 Russia.
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Mol Biol (Mosk). 2020 Jan-Feb;54(1):146-152. doi: 10.31857/S0026898420010176.
Migration of cancer cells from the primary tumor site to nearby tissues is the starting point of the metastatic process. The invasive properties of cells are especially important for carcinomas, since tumor cells need to overcome the basement membrane and go beyond its boundaries to the underlying tissues. Substances that reduce the invasive ability of malignant cells are promising as antimetastatic agents. In the present work, the possibility of inhibiting the ability of different cancer cell lines to migrate under the influence of the Bacillus pumilus ribonuclease (binase) was analyzed using the scratch-wound assay. It was established that binase at non-toxic concentrations (10 μg/mL) reliably suppressed the migratory ability of HuTu 80 human duodenum adenocarcinoma cells incubated with RNase for 48-72 h. The antimetastatic potential of binase is confirmed by molecular modeling data demonstrating the ability of binase to inhibit cellular metalloproteinases that determine the migration of tumor cells.
癌细胞从原发性肿瘤部位迁移至附近组织是转移过程的起点。细胞的侵袭特性对于癌尤其重要，因为肿瘤细胞需要突破基底膜并超越其边界进入下方组织。降低恶性细胞侵袭能力的物质有望成为抗转移剂。在本研究中，使用划痕试验分析了短小芽孢杆菌核糖核酸酶（binase）影响下不同癌细胞系迁移能力受到抑制的可能性。结果表明，无毒浓度（10μg/mL）的binase能可靠地抑制与核糖核酸酶孵育48 - 72小时的HuTu 80人十二指肠腺癌细胞的迁移能力。分子模拟数据证实了binase的抗转移潜力，该数据表明binase能够抑制决定肿瘤细胞迁移的细胞金属蛋白酶。
