Torresi Claudia, Fiori Mariangela, Bertolotti Luigi, Floris Matteo, Colitti Barbara, Giammarioli Monica, Dei Giudici Silvia, Oggiano Annalisa, Malmberg Maja, De Mia Gian Mario, Belák Sándor, Granberg Fredrik
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati" (IZS-UM), Perugia, Italy.
Istituto Zooprofilattico Sperimentale della Sardegna (IZS-SA), Sassari, Italy.
Transbound Emerg Dis. 2020 Sep;67(5):1971-1980. doi: 10.1111/tbed.13540. Epub 2020 Mar 23.
African swine fever (ASF) is a highly contagious and lethal viral disease of pigs and wild boars, which is enzootic in many African countries and on the Italian island of Sardinia, where it has been present since 1978. Previous genetic analyses of Sardinian ASF virus (ASFV) isolates have revealed that they all belong to p72 genotype I, with only minor sequence variations. However, these studies examined only a few selected genes. To distinguish between these closely related isolates and better investigate ASFV evolution in Sardinia, we sequenced the complete genomes of 12 Sardinian ASFV isolates collected between 1978 and 2012, and compared them with 47/Ss/2008 and 26544/OG10. Most of the observed changes occurred in a time-dependent manner; however, their biological significance remains unclear. As a whole, our results demonstrate the remarkable genetic stability of these strains, supporting a single-source introduction of the virus.
非洲猪瘟（ASF）是一种猪和野猪的高度传染性致命病毒性疾病，在许多非洲国家以及意大利撒丁岛呈地方流行性，自1978年以来该病毒就在撒丁岛存在。此前对撒丁岛非洲猪瘟病毒（ASFV）分离株的基因分析显示，它们均属于p72基因型I，仅有微小的序列变异。然而，这些研究仅检测了少数几个选定的基因。为了区分这些密切相关的分离株，并更好地研究撒丁岛ASFV的进化，我们对1978年至2012年间收集的12株撒丁岛ASFV分离株的完整基因组进行了测序，并将它们与47/Ss/2008和26544/OG10进行了比较。观察到的大多数变化呈时间依赖性发生；然而，它们的生物学意义仍不清楚。总体而言，我们的结果证明了这些毒株具有显著的遗传稳定性，支持该病毒为单源引入。
