Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, 11944, USA.
Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, USDA, Greenport, NY, 11944, USA.
Sci Rep. 2023 Jan 19;13(1):1024. doi: 10.1038/s41598-022-25987-5.
African swine fever is a lethal disease of domestic pigs, geographically expanding as a pandemic, that is affecting countries across Eurasia and severely damaging their swine production industry. After more than 40 years of being absent in the Western hemisphere, in 2020 ASF reappeared in the Dominican Republic and Haiti. The recent outbreak strain in the Dominican Republic has been identified as a genotype II ASFV a derivative of the ASF strain circulating in Asia and Europe. However, to date no full-length genome sequence from either the 1978-1980 Here we report the complete genome sequence of an African swine fever virus (ASFV) (DR-1980) that was previously isolated from blood collected in 1980 from the Dominican Republic at the end of the last outbreak, before culling of all swine on the island of Hispaniola and stored in the Plum Island Animal Disease Center ASFV repository. A contig representing the full-length genome (183,687 base pairs) was de novo assembled into a single contig using both Nanopore and Illumina sequences. DR-1980 was determined to belong to genotype I and, as determined by full genome comparison, a close relative to the sequenced Sardinia viruses that were causing outbreaks at this time.
非洲猪瘟是一种致命的家猪疾病，呈地理性蔓延趋势，已在欧亚大陆的多个国家流行，严重损害了这些国家的养猪业。在西半球消失 40 多年后，2020 年非洲猪瘟再次出现在多米尼加共和国和海地。最近在多米尼加共和国爆发的疫情株被确定为基因型 II 的 ASFV，它是一种源自亚洲和欧洲流行的 ASFV 株的衍生物。然而，截至目前，尚未从 1978-1980 年在该岛国上宰杀所有猪之前采集的、来自多米尼加共和国的血液中分离到的任何全长基因组序列。本研究中，我们报告了一种非洲猪瘟病毒（ASFV）（DR-1980）的完整基因组序列，该病毒是在 1980 年最后一次疫情结束时从多米尼加共和国采集的血液中分离出来的，当时该病毒已在伊斯帕尼奥拉岛被宰杀并储存在普林斯顿岛动物疾病中心的 ASFV 储存库中。使用 Nanopore 和 Illumina 序列，从头组装了一个代表全长基因组（183687 个碱基对）的重叠群。DR-1980 被确定属于基因型 I，通过全基因组比较，它与此时引起暴发的已测序的撒丁岛病毒密切相关。
