Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 5;233:118234. doi: 10.1016/j.saa.2020.118234. Epub 2020 Mar 5.
Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.
次氯酸在生物系统中发挥了多种功能。然而，过量的 HOCl 会对生物分子造成损伤，导致一些疾病。因此，人们开发了一种新的荧光探针 BSP，通过 HOCl 诱导的甲基苯硫醚氧化为亚砜来快速识别 HOCl。BSP 与 HOCl 的反应导致荧光增强了 22 倍（量子产率从 0.006 增加到 0.133）。发现 HOCl 的检测限为 30 nM（S/N=3）。荧光增强是由于抑制了从甲基苯硫醚部分到 BODIPY 的光致电子转移。最终，细胞荧光成像实验表明，BSP 可有效用于监测活细胞中的 HOCl。
