Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Apr 1;1142:122039. doi: 10.1016/j.jchromb.2020.122039. Epub 2020 Feb 22.
The present study included the procedure of preparing porous titania thin film using a direct nanocrystalline cellulose templating (NCC) as a bio-template. The microextraction applicability of the porous film was investigated by thin film microextraction (TFME) of the nonsteroidal anti-inflammatory drugs (NSAIDs) including ketorolac, meloxicam, diclofenac and mefenamic acid from different types of urine sample. The extracted NSAIDs were analyzed by HPLC-UV. Under optimum conditions, the calibration curves were linear within the range of 1.0-500 µg L (2.0-200 µg L for ketorolac, 2.0-500 µg L for meloxicam, 1.0-200 µg L for diclofenac and 1.0-200 µg L for mefenamic acid). The limit of detection was found to be between 0.2 and 0.5 µg L. The calculated intra- and inter-day relative standard deviations RSDs% (n = 3) at concentration level of 10 µg L were less than 6.3% and 6.0%, respectively. Finally, the method was successfully applied to determine selected NSAIDs in urine samples from different human individuals.
本研究采用直接纳米纤维素模板（NCC）作为生物模板，制备了多孔二氧化钛薄膜。通过薄膜微萃取（TFME）从不同类型的尿液样本中萃取非甾体抗炎药（NSAIDs），如酮咯酸、美洛昔康、双氯芬酸和甲芬那酸，研究了多孔薄膜的微萃取适用性。用 HPLC-UV 分析提取的 NSAIDs。在最佳条件下，校准曲线在 1.0-500 µg L 范围内呈线性（酮咯酸为 2.0-200 µg L，美洛昔康为 2.0-500 µg L，双氯芬酸为 1.0-200 µg L，甲芬那酸为 1.0-200 µg L）。检测限发现介于 0.2 和 0.5 µg L 之间。在 10 µg L 的浓度水平下，计算得到的日内和日间相对标准偏差（RSD）%（n=3）分别小于 6.3%和 6.0%。最后，该方法成功应用于测定来自不同个体的尿液样本中选定的 NSAIDs。
