对蝗虫体型进行人工选择后的转录组分析。

Transcriptomic Analysis Following Artificial Selection for Grasshopper Size.

作者信息

Li Shuang, Cui Dong-Nan, Ullah Hidayat, Chen Jun, Liu Shao-Fang, Whitman Douglas W, Zhang Ze-Hua, Tu Xiong-Bing

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Department of Agriculture, The University of Swabi, Anbar 23561, Khyber Pakhtunkhwa, Pakistan.

出版信息

Insects. 2020 Mar 10;11(3):176. doi: 10.3390/insects11030176.

DOI:10.3390/insects11030176

PMID:32164277

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7142927/

Abstract

We analyzed the transcriptomes of grasshoppers after 8 years of artificial selection for either long or short thoraces. Evolution proceeded rapidly during the experiment, with a 13.3% increase and a 32.2% decrease in mean pronotum lengths (sexes combined) in the up- and down-selected colonies, respectively, after only 11 generations. At least 16 additional traits also diverged between the two colonies during the selection experiment. Transcriptomic analysis identified 693 differentially expressed genes, with 386 upregulated and 307 downregulated (55.7% vs. 44.3%), including cellular process, metabolic process, binding, general function prediction only, and signal transduction mechanisms. Many of the differentially expressed genes (DEGs) are known to influence animal body size.

摘要

我们分析了经过8年针对长胸或短胸进行人工选择的蝗虫的转录组。在实验过程中，进化进展迅速，仅经过11代，在向上和向下选择的群体中，平均前胸背板长度（两性合并）分别增加了13.3%和减少了32.2%。在选择实验期间，两个群体之间至少还有16个其他性状也出现了分化。转录组分析鉴定出693个差异表达基因，其中386个上调，307个下调（55.7%对44.3%），包括细胞过程、代谢过程、结合、仅一般功能预测和信号转导机制。许多差异表达基因已知会影响动物体型。