Spenger C, Haque N S, Studer L, Evtouchenko L, Wagner B, Bühler B, Lendahl U, Dunnett S B, Seiler R W
Department of Neurosurgery, University of Bern, Inselspital, Switzerland.
Exp Brain Res. 1996 Nov;112(1):47-57. doi: 10.1007/BF00227177.
Free-floating roller tube cultures of human fetal (embryonic age 6-10 weeks post-conception) and rat fetal (embryonic day 13) ventral mesencephalon were prepared. After 7-15 days in vitro, the mesencephalic tissue cultures were transplanted into the striatum of adult rats that had received unilateral injections of 6-hydroxydopamine into the nigrostriatal bundle 3-5 weeks prior to transplantation. Graft survival was assessed in tyrosine hydroxylase (TH)-immunostained serial sections of the grafted brains up to post-transplantation week 4 for the human fetal xenografts and post-transplantation week 11 for the rat fetal allografts. D-amphetamine-induced rotation was monitored up to 10 weeks after transplantation in the allografted animals and compared with that of lesioned-only control animals. All transplanted animals showed large, viable grafts containing TH-immunoreactive (ir) neurons. The density of TH-ir neurons in the human fetal xenografts and in rat fetal allografts was similar. A significant amelioration of the amphetamine-induced rotation was observed in the animals that received cultured tissue allografts. These results promote the feasibility of in vitro maintenance of fetal human and rat nigral tissue prior to transplantation using the free-floating roller tube technique.
制备了人胎儿（受孕后6 - 10周胚胎龄）和大鼠胎儿（胚胎第13天）腹侧中脑的游离滚动管培养物。体外培养7 - 15天后，将中脑组织培养物移植到成年大鼠的纹状体中，这些成年大鼠在移植前3 - 5周已单侧向黑质纹状体束注射6 - 羟基多巴胺。在移植后第4周对人胎儿异种移植的移植脑酪氨酸羟化酶（TH）免疫染色连续切片进行评估，对大鼠胎儿同种移植的移植脑在移植后第11周进行评估，以评估移植物存活情况。在同种移植动物中监测移植后长达10周的右旋苯丙胺诱导的旋转，并与仅损伤的对照动物进行比较。所有移植动物均显示含有TH免疫反应性（ir）神经元的大的、存活的移植物。人胎儿异种移植和大鼠胎儿同种移植中TH - ir神经元的密度相似。在接受培养组织同种移植的动物中观察到右旋苯丙胺诱导的旋转有显著改善。这些结果表明使用游离滚动管技术在移植前体外维持胎儿人及大鼠黑质组织的可行性。
