Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.
School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Anal Chem. 2020 Apr 7;92(7):4990-4995. doi: 10.1021/acs.analchem.9b05149. Epub 2020 Mar 20.
Rapid, sensitive, and reliable pathogen detection is growing in importance in human health and safety. In this work, we report a stochastic DNA dual-walker-based colorimetric biosensor for bacterial detection. In the presence of target bacteria, two kinds of released multiple walking strands are allowed for continuous walking on the Au nanoparticle (AuNP)-based 3D track, resulting in destabilized aggregation of AuNP-based probes. The induced color change from red to blue can serve as an analytical signal for colorimetric detection of target bacteria. We demonstrated that this mothed enables sensitive and specific bacterial detection within 15 min due to its ultrafast reaction kinetics and sensitive color change, showing a linear response ranging from 10 to 10 CFU/mL with a limit of detection of 1 CFU/mL. Moreover, we also realized analysis of practical samples using this colorimetric biosensor. Given its features of rapid, sensitive, specific, and reliable analysis, our stochastic dual-walker-based colorimetric biosensor shows much promise in point-of-care testing for bacteria detection.
快速、灵敏、可靠的病原体检测在人类健康和安全方面的重要性日益增加。在这项工作中，我们报告了一种基于随机 DNA 双游走体的比色生物传感器，用于细菌检测。在存在靶细菌的情况下，两种释放的多游走链被允许在基于金纳米粒子（AuNP）的 3D 轨道上连续行走，导致基于 AuNP 的探针的不稳定性聚集。诱导的颜色从红色变为蓝色可作为比色检测靶细菌的分析信号。我们证明，由于其超快的反应动力学和灵敏的颜色变化，该方法能够在 15 分钟内实现灵敏和特异的细菌检测，显示出从 10 到 10 CFU/mL 的线性响应，检测限为 1 CFU/mL。此外，我们还使用这种比色生物传感器实现了实际样品的分析。鉴于其快速、灵敏、特异和可靠分析的特点，我们基于随机双游走体的比色生物传感器在即时检测细菌检测方面具有广阔的应用前景。
