Department of Clinical Medicine, Weifang Medical University, Weifang, Shandong, China.
Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital affiliated to Shandong University, Jinan, Shandong, China.
Exp Lung Res. 2020 Apr-May;46(3-4):98-108. doi: 10.1080/01902148.2020.1740356. Epub 2020 Mar 12.
Airway remodeling is one of the features of severe asthma. Previous study shows that IL-27 inhibits airway inflammation in asthmatic mice. However, the role of IL-27 on airway remodeling in OVA-induced asthmatic mice and its possible mechanism remain unclear. We established an ovalbumin (OVA)-induced asthmatic mice model. IL-27 were preventative administered to OVA-induced asthmatic mice. The total cells in Bronchoalveolar lavage fluid (BALF) and Airway hyperresponsiveness (AHR) were measured. The lung tissues were performed by Hematoxylin and eosin (HE) staining to estimate the pathological changes. Masson staining was used to observe the collagen deposition area. The expression of α-smooth muscle actin (α-SMA) and Type I collagen was measured by immunohistochemistry, western blot, and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Additionally, western blot was also used to measure the expression of phosphorylated-Akt (p-Akt) in each group. IL-27 group showed significant inhibitory effect on the α-SMA and Type I collagen. The expression of p-Akt in the tissues of asthma model was increased and inhibited by IL-27. IL-27 can alleviate airway remodeling in OVA-induced asthmatic mice, and the mechanism may relate to PI3K/Akt pathway.
气道重塑是严重哮喘的特征之一。先前的研究表明，IL-27 可抑制哮喘小鼠的气道炎症。然而，IL-27 对卵清蛋白（OVA）诱导的哮喘小鼠气道重塑的作用及其可能的机制尚不清楚。我们建立了卵清蛋白（OVA）诱导的哮喘小鼠模型。IL-27 预防性给予 OVA 诱导的哮喘小鼠。测量支气管肺泡灌洗液（BALF）中的总细胞数和气道高反应性（AHR）。通过苏木精和伊红（HE）染色对肺组织进行评估以估计病理变化。Masson 染色用于观察胶原沉积区。通过免疫组织化学、Western blot 和定量逆转录聚合酶链反应（qRT-PCR）测量α-平滑肌肌动蛋白（α-SMA）和 I 型胶原的表达。此外，Western blot 还用于测量各组中磷酸化-Akt（p-Akt）的表达。IL-27 组对 α-SMA 和 I 型胶原表现出显著的抑制作用。哮喘模型组织中 p-Akt 的表达增加，并被 IL-27 抑制。IL-27 可减轻 OVA 诱导的哮喘小鼠的气道重塑，其机制可能与 PI3K/Akt 通路有关。
