Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Chennai campus, Vandalur-Kelambakkam Road, Chennai - 600 127, Tamil Nadu, India.
Endocr Metab Immune Disord Drug Targets. 2020;20(7):1097-1109. doi: 10.2174/1871530320666200312152331.
Clerodendrum paniculatum has ethnomedicinal importance in treatment of disorders like wound, typhoid, jaundice, malaria and anemia.
To evaluate the antioxidant and hepatoprotective activity of Clerodendrum paniculatum leaves against carbon tetrachloride (CCl4) induced rat model and identification of its bioactive constituents by Gas Chromatography Mass Spectroscopy (GC MS).
Successive solvent extraction was carried out. Total phenolic, flavonoid content and antioxidant activity by 2,2- diphenyl-1-picryl hydrazyl (DPPH), nitric oxide and 2-Azino-bis [3-ethyl benzothiazoline- 6-sufonic acid] (ABTS method) were done. Ethyl acetate extract was selected for hepatoprotective study in carbon tetrachloride intoxicated model followed by the measurement of liver function marker enzymes such as SGOT (Serum Glutamate Oxaloacetate Transaminase), SGPT (Serum Glutamate Pyruvate Transaminase), and ALP (Alkaline Phosphatase). Biochemical parameters like bilirubin and protein were measured. Histopathologic liver sections were carried out. Bioactive constituents were evaluated by GC MS.
By DPPH and ABTS method, ethyl acetate extract showed IC50 as 70.14±0.92 μg/ml,2958.24±2.460 μg/ml, respectively. The alcoholic extract showed maximum IC50 (197.22 ±7.16 μg/ml) by Nitric oxide radical scavenging method. Hepatoprotective study reveals that intoxicated animal groups have elevated levels of enzymes and bilirubin and suppress the production of protein. The extract pre-treatment showed a significant decrease in enzymes and increased production of total protein in a dose-dependent manner. Histopathologic studies also support the hepatoprotective activity. GC MS analysis revealed the presence of seven major bioactive constituents with ethyl palmitate as the major one.
The results support the proof for the hepatoprotective potential of the CPLE extract with potent antioxidant activity and enhanced liver enzyme level. The observed activity could be due to the presence of bioactive compounds as identified by GC MS analysis.
臭牡丹在治疗创伤、伤寒、黄疸、疟疾和贫血等疾病方面具有民族医学重要性。
评估臭牡丹叶对四氯化碳（CCl4）诱导的大鼠模型的抗氧化和保肝活性，并通过气相色谱-质谱联用（GC-MS）鉴定其生物活性成分。
进行连续溶剂提取。通过 2,2-二苯基-1-苦基肼基（DPPH）、一氧化氮和 2-氮杂双-[3-乙基苯并噻唑啉-6-磺酸]（ABTS 法）测定总酚、类黄酮含量和抗氧化活性。选择乙酸乙酯提取物进行碳四氯化碳中毒模型的保肝研究，然后测定血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）和碱性磷酸酶（ALP）等肝功能标记酶。测定胆红素和蛋白质等生化参数。进行肝组织病理学切片检查。通过 GC-MS 评估生物活性成分。
通过 DPPH 和 ABTS 法，乙酸乙酯提取物的 IC50 分别为 70.14±0.92μg/ml 和 2958.24±2.460μg/ml。乙醇提取物通过一氧化氮自由基清除法显示出最大的 IC50（197.22 ±7.16μg/ml）。保肝研究表明，中毒动物组的酶和胆红素水平升高，蛋白质生成受到抑制。提取物预处理以剂量依赖性方式显著降低酶并增加总蛋白的产生。组织病理学研究也支持其保肝活性。GC-MS 分析显示存在七种主要生物活性成分，其中以乙基棕榈酸酯为主。
结果支持 CPLE 提取物具有潜在的抗氧化和增强肝脏酶水平的保肝作用的证据。观察到的活性可能是由于 GC-MS 分析鉴定的生物活性化合物的存在。
