Daulay Milahayati, Sari Mutiara Indah, Wahyuni Dian Dwi, Syarifah Siti
Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Jl. dr. Mansur Kampus USU Medan 20155, Indonesia.
Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Jl. dr. Mansur Kampus USU Medan 20155, Indonesia.
Open Access Maced J Med Sci. 2019 Dec 10;7(23):3960-3964. doi: 10.3889/oamjms.2019.824. eCollection 2019 Dec 15.
The gene polymorphism (-308G/A) and tumor necrosis factor-alpha (TNF-α) levels influence development of disease in type 2 diabetic patients and tuberculosis patients.
In this study, we analyze the association between the TNF-α polymorphisms (-308G/A) and the levels of TNF-α in type 2 diabetic patients with and without tuberculosis infection.
This study was an analytic observational with cross sectional approach consisting 40 type 2 diabetic patients with tuberculosis infection, 40 type 2 diabetic patients without tuberculosis infection and 40 healthy control (HC) subjects. The TNF-α gene polymorphism (-308G/A) was analyzed with polymerase chain reaction-restriction fragment lengths polymorphisms (PCR-RFLP) method. The TNF-α levels were measured using an enzyme-linked immunosorbent assay. The association between gene polymorphism (-308G/A) in study groups was analyzed by Fisher's exact test, tumor necrosis factor-alpha (TNF-α) levels in study groups was carried out using the Kruskal-Wallis test. Hardy-Weinberg Equilibrium also determined genotype deviation and allele frequencies.
The GG and GA+AA genotypes frequency in both of patient groups and HC subjects were not differ significantly (95% and 5% vs 95% and 5% vs 92.5% and 7.5%; p > 0.05). The TNF-α levels (pg/ml) of type 2 diabetic without tuberculosis infection were higher than those of type 2 diabetic with tuberculosis infection and HC subjects (7.42 ± 0.78 vs 2.23 ± 0.51 vs 2.57 ± 0.63; p < 0.01). The TNF-α levels in the GA+AA genotypes were higher than the GG wild-type genotype (p > 0.05). There was no significant deviation of genotype frequency and allele from Hardy-Weinberg Equilibrium.
The gene polymorphism (-308G/A) had no association with type 2 diabetic patients with and without tuberculosis infection and the gene polymorphism (-308G/A) was not influence the TNF-α levels but there was a significant differentiation of TNF-α levels between the groups.
基因多态性（-308G/A）和肿瘤坏死因子-α（TNF-α）水平影响2型糖尿病患者和结核病患者的疾病发展。
在本研究中，我们分析2型糖尿病合并或未合并结核感染患者中TNF-α基因多态性（-308G/A）与TNF-α水平之间的关联。
本研究为横断面分析观察性研究，包括40例合并结核感染的2型糖尿病患者、40例未合并结核感染的2型糖尿病患者和40例健康对照（HC）受试者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析TNF-α基因多态性（-308G/A）。使用酶联免疫吸附测定法测量TNF-α水平。研究组间基因多态性（-308G/A）的关联采用Fisher精确检验分析，研究组中肿瘤坏死因子-α（TNF-α）水平采用Kruskal-Wallis检验。哈迪-温伯格平衡也用于确定基因型偏差和等位基因频率。
两组患者和HC受试者中GG和GA+AA基因型频率无显著差异（95%和5%对95%和5%对92.5%和7.5%；p>0.05）。未合并结核感染的2型糖尿病患者的TNF-α水平（pg/ml）高于合并结核感染的2型糖尿病患者和HC受试者（7.42±0.78对2.23±0.51对2.57±0.63；p<0.01）。GA+AA基因型中的TNF-α水平高于GG野生型基因型（p>0.05）。基因型频率和等位基因与哈迪-温伯格平衡无显著偏差。
基因多态性（-308G/A）与合并或未合并结核感染的2型糖尿病患者无关，基因多态性（-308G/A）不影响TNF-α水平，但各组间TNF-α水平存在显著差异。
