Mehrvar Shima, Rymut Kevin T, Foomani Farnaz H, Mostaghimi Soudeh, Eells Janis T, Ranji Mahsa, Gopalakrishnan Sandeep
1Biophotonics LabDepartment of Electrical EngineeringUniversity of Wisconsin MilwaukeeMilwaukeeWI53211USA.
2College of NursingUniversity of Wisconsin MilwaukeeMilwaukeeWI53211USA.
IEEE J Transl Eng Health Med. 2019 Oct 18;7:1800809. doi: 10.1109/JTEHM.2019.2945323. eCollection 2019.
Diabetes is known to cause delayed wound healing, and chronic non-healing lower extremity ulcers may end with lower limb amputations and mortalities. Given the increasing prevalence of diabetes mellitus worldwide, it is critical to focus on underlying mechanisms of these debilitating wounds to find novel therapeutic strategies and thereby improve patient outcome. This study aims to design a label-free optical fluorescence imager that captures metabolic indices (NADH and FAD autofluorescence) and monitors the wound healing progress noninvasively. Furthermore, 3D optical cryo-imaging of the mitochondrial redox state was utilized to assess the volumetric redox state of the wound tissue. The results from our fluorescence imager and the 3D cryo-imager quantify the differences between the redox state of wounds on diabetic mice in comparison with the control mice. These metabolic changes are associated with mitochondrial dysfunction and higher oxidative stress in diabetic wounds. A significant correlation was observed between the redox state and the area of the wounds. The results suggest that our developed novel optical imaging system can successfully be used as an optical indicator of the complex wound healing process noninvasively.
众所周知，糖尿病会导致伤口愈合延迟，慢性不愈合的下肢溃疡可能会导致下肢截肢和死亡。鉴于全球糖尿病患病率不断上升，关注这些致残性伤口的潜在机制以寻找新的治疗策略从而改善患者预后至关重要。本研究旨在设计一种无标记光学荧光成像仪，该成像仪可捕捉代谢指标（NADH和FAD自发荧光）并无创监测伤口愈合进程。此外，利用线粒体氧化还原状态的三维光学冷冻成像来评估伤口组织的体积氧化还原状态。我们的荧光成像仪和三维冷冻成像仪的结果量化了糖尿病小鼠伤口与对照小鼠伤口氧化还原状态之间的差异。这些代谢变化与糖尿病伤口中的线粒体功能障碍和更高的氧化应激有关。氧化还原状态与伤口面积之间存在显著相关性。结果表明，我们开发的新型光学成像系统可以成功地用作无创监测复杂伤口愈合过程的光学指标。
