3D 光学冷冻成像方法：一种定量分析肾脏线粒体生物能量障碍的新方法。

3D Optical Cryo-Imaging Method: A Novel Approach to Quantify Renal Mitochondrial Bioenergetics Dysfunction.

机构信息

University of Wisconsin-Milwaukee, Milwaukee, WI, USA.

Department of Anesthesiology and Anesthesia Research, Medical College of Wisconsin, Wauwatosa, WI, USA.

出版信息

Methods Mol Biol. 2021;2276:259-270. doi: 10.1007/978-1-0716-1266-8_20.

DOI:10.1007/978-1-0716-1266-8_20

PMID:34060048

Abstract

Mitochondrial dysfunction contributes to various injuries and diseases. A mechanistic understanding of how dysfunctional mitochondria modulates metabolism is of paramount importance. Three-dimensional (3D) optical cryo-imager is a custom-designed device that can quantify the volumetric bioenergetics of organs in small animal models. The instrument captures the autofluorescence of bioenergetics indices (NADH and FAD) from tissues at cryogenic temperature. The quantified redox ratio (NADH/FAD) is used as an optical indicator of mitochondrial redox state.

摘要

线粒体功能障碍与各种损伤和疾病有关。深入了解功能失调的线粒体如何调节代谢至关重要。三维（3D）光学冷冻成像仪是一种定制设计的设备，可定量测量小动物模型中器官的容积生物能量学。该仪器在低温下捕获生物能量学指标（NADH 和 FAD）的自发荧光。量化的氧化还原比（NADH/FAD）用作线粒体氧化还原状态的光学指示剂。