Fatolahi Hoseyn, Azarbayjani Mohamad Ali, Peeri Maghsoud, Matinhomaei Hasan
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Clin Exp Hepatol. 2020 Feb;6(1):49-54. doi: 10.5114/ceh.2020.93057. Epub 2020 Feb 17.
Binge ethanol drinking causes liver damage and decreased paraoxonase-1 (PON-1) gene expression. On the other hand, regular physical activity and curcumin consumption as non-invasive interventions can have liver protective effects through enhancing antioxidant defense, and improving PON-1 and NF-kβ (nuclear factor kappa B) gene expression. The aim of this study was to investigate the interactive effect of exercise rehabilitation and curcumin consumption on hepatocyte damage as well as NF-kβ and PON-1 gene expression in rats.
Fifty-six male Wistar rats were randomly selected and equally divided into seven groups: dextrose-control (Dext-Con), ethanol-control (Eth-Con), ethanol-saline (Eth-sal), ethanol-DMSO (Eth-DMSO), ethanol-curcumin (Eth-Cur), ethanol-swimming training (Eth-SWT) and ethanol-SWT + curcumin (Eth-SWT + Cur). After four days of the binge drinking protocol followed by six days of quitting, the interventions of SWT and curcumin (50 mg/kg) were employed for 14 days. Afterwards, the rats' liver tissues were collected and sent to the laboratory for biochemical assays.
The interaction of SWT and curcumin caused an increase in PON-1 gene expression ( = 0.02). In addition, curcumin consumption ( = 0.003) and its interaction with SWT ( = 0.004) resulted in a reduction in NF-kβ gene expression. Also, liver tissue damage was observed in the Eth-Con group compared to other groups.
The combination of curcumin and SWT may be used to reduce the side effects of binge ethanol drinking and improve recovery in the quitting period.
暴饮乙醇会导致肝损伤并降低对氧磷酶-1（PON-1）基因表达。另一方面，规律的体育活动和食用姜黄素作为非侵入性干预措施，可通过增强抗氧化防御以及改善PON-1和NF-κβ（核因子κB）基因表达而具有肝脏保护作用。本研究的目的是探讨运动康复和食用姜黄素对大鼠肝细胞损伤以及NF-κβ和PON-1基因表达的交互作用。
随机选取56只雄性Wistar大鼠，将其平均分为七组：葡萄糖对照组（Dext-Con）、乙醇对照组（Eth-Con）、乙醇-生理盐水组（Eth-sal）、乙醇-二甲基亚砜组（Eth-DMSO）、乙醇-姜黄素组（Eth-Cur）、乙醇-游泳训练组（Eth-SWT）和乙醇-游泳训练+姜黄素组（Eth-SWT + Cur）。在进行四天暴饮方案并随后戒酒六天后，采用游泳训练和姜黄素（50毫克/千克）干预14天。之后，收集大鼠肝脏组织并送至实验室进行生化检测。
游泳训练和姜黄素的交互作用使PON-1基因表达增加（P = 0.02）。此外，食用姜黄素（P = 0.003）及其与游泳训练的交互作用（P = 0.004）导致NF-κβ基因表达降低。而且，与其他组相比，在乙醇对照组中观察到肝脏组织损伤。
姜黄素与游泳训练相结合可用于减轻暴饮乙醇的副作用并改善戒酒期的恢复情况。
