Bagés-Arnal Sandra, Sánchez José M, Fernandez-Fuertes Beatriz, McDonald Michael, Behura Susanta K, Spencer Thomas E, Fair Trudee, Lonergan Pat
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
Reproduction. 2020 May;159(5):643-657. doi: 10.1530/REP-19-0464.
In cattle, embryo transfer into the uterine horn contralateral to the corpus luteum results in a higher incidence of pregnancy loss compared to transfer into the ipsilateral horn. We have previously reported temporal changes in the endometrial transcriptome during the estrous cycle which differ between uterine horns. The objective of this study was to compare the transcriptomic response of endometrium from the ipsilateral and contralateral horns to an elongating conceptus. Cross-bred beef heifers (n = 16) were synchronized and either used to generate day 14 conceptuses following the transfer of in vitro-produced blastocysts or to obtain day 14 endometrial explants. Conceptuses were recovered on day 14 by post-mortem uterine flushing, placed individually on top of explants collected from the ipsilateral (IPSI-D14) or the contralateral (CONTRA-D14) uterine horn of cyclic heifers, and co-cultured for 6 h. The response to a conceptus was markedly different between uterine horns, with 61 and 239 differentially expressed genes (DEGs; false discovery rate <0.05) in the ipsilateral and contralateral horns, respectively, compared to their controls. Direct comparison between IPSI-D1 and CONTRA-D14 revealed 32 DEGs, including CXCL11, CXCL10, IFIT2, RSAD2 and SAMD9. Gene Ontology analysis of these 32 genes revealed ten enriched biological processes, mainly related to immune response and response to an external stimulus. These data indicate that the endometrial response to the presence of a conceptus varies between uterine horns in the same uterus and may contribute to the higher incidence of pregnancy loss following embryo transfer to the contralateral horn.
在牛中，与将胚胎移植到黄体同侧子宫角相比，将胚胎移植到黄体对侧子宫角会导致更高的妊娠丢失率。我们之前报道过发情周期中子宫内膜转录组的时间变化，子宫角之间存在差异。本研究的目的是比较同侧和对侧子宫角的子宫内膜对伸长中的孕体的转录组反应。对16头杂交肉用小母牛进行同期发情处理，然后将体外生产的囊胚移植后用于生成第14天的孕体，或用于获取第14天的子宫内膜外植体。在第14天通过死后子宫冲洗回收孕体，将其分别置于从同期发情小母牛的同侧（IPSI-D14）或对侧（CONTRA-D14）子宫角收集的外植体上，并共同培养6小时。子宫角之间对孕体的反应明显不同，与各自的对照相比，同侧和对侧子宫角分别有61个和239个差异表达基因（DEG；错误发现率<0.05）。IPSI-D14和CONTRA-D14之间的直接比较显示有32个DEG，包括CXCL11、CXCL10、IFIT2、RSAD2和SAMD9。对这32个基因的基因本体分析揭示了10个富集的生物学过程，主要与免疫反应和对外界刺激的反应有关。这些数据表明，同一子宫内子宫角之间子宫内膜对孕体存在的反应不同，这可能是胚胎移植到对侧子宫角后妊娠丢失率较高的原因。
