Bolton R W, Rozmiarek H, Chorpenning F W
J Immunol. 1977 Apr;118(4):1154-8.
Normal adult Sprague-Dawley rats were bled serially over a 30-week period and their sera were examined for antibodies to polyglycerophosphate (PGP) by a standardized passive hemolysis test. Levels of "natural" antibodies to PGP fluctuated during this period with a majority of animals exhibiting pronounced cycling of serum antibody levels, however, the individual cycles were not synchronized with each other. Feeding of radiolabeled Gram-positive bacilli to these animals and examination of lymphoid tissue by liquid scintillation counting revealed that the antigen persisted mainly in the mesenteric lymph nodes. A second group of rats was injected i.v. with radiolabeled Gram-positive bacilli and tissues were examined for plaque-forming cells (PFC) of PGP specificity, and the sera were examined by passive hemolysis. Cycling of both anti-PGP antibodies and PFC became synchronized in the injected animals with peaks of serum antibody evident at 16 and 28 days post-injection and splenic PFC peaks at 4 and 16 days post-injection. Cycling was also observed in the mesenteric lymph nodes and bone marrow Examination of lymphoid tissue from the rats injected i.v. revealed that antigen introduced by this route also perisisted mainly in the mesenteric lymph nodes, This report demonstrates individual cycling of natural responses to environmental antigen and to the same determinant in secondary responses, indicating its importance as a regulatory mechanism.
正常成年Sprague-Dawley大鼠在30周内连续采血，通过标准化被动溶血试验检测其血清中针对聚甘油磷酸（PGP）的抗体。在此期间，PGP的“天然”抗体水平波动，大多数动物血清抗体水平呈现明显的周期性变化，然而，各个周期并不相互同步。给这些动物喂食放射性标记的革兰氏阳性杆菌，并通过液体闪烁计数检查淋巴组织，结果显示抗原主要存在于肠系膜淋巴结中。第二组大鼠经静脉注射放射性标记的革兰氏阳性杆菌，检查具有PGP特异性的空斑形成细胞（PFC），并通过被动溶血检测血清。在注射的动物中，抗PGP抗体和PFC的周期性变化变得同步，注射后16天和28天血清抗体出现峰值，脾脏PFC在注射后4天和16天出现峰值。在肠系膜淋巴结和骨髓中也观察到了周期性变化。对经静脉注射的大鼠的淋巴组织检查显示，通过该途径引入的抗原也主要存在于肠系膜淋巴结中。本报告证明了对环境抗原的天然反应以及二次反应中对相同决定簇的反应存在个体周期性变化，表明其作为一种调节机制的重要性。
