• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

NEAT1 对于肝癌干细胞标志物 CD44 的表达是必需的。

NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44.

机构信息

Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University, 86 Nishi-cho, Yonago 683-8503, Japan.

Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago 683-8503, Japan.

出版信息

Int J Mol Sci. 2020 Mar 11;21(6):1927. doi: 10.3390/ijms21061927.

DOI:10.3390/ijms21061927
PMID:32168951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7139689/
Abstract

CD44, a cancer stem cell (CSC) marker, is required for maintaining CSC properties in hepatocellular carcinoma (HCC). Nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), is an oncogenic driver in HCC. In the present study, we investigated the significance of the gene in association with CD44 expression in liver CSCs of human HCC cell lines. The CSC properties were evaluated by spheroid culture, CSC marker expression, and sensitivity to anti-cancer drugs. The expression of both NEAT1 variant 1 (NEAT1v1) and variant 2 (NEAT1v2) as well as CD44 was significantly increased in the spheroid culture, compared with that in monolayer culture. Overexpression of Neat1v1, but not Neat1v2, enhanced the CSC properties, while knockout of the NEAT1 gene suppressed them. CD44 expression was increased by the overexpression of Neat1v1 and abrogated by NEAT1 knockout. The overexpression of NEAT1v1 restored the CSC properties and CD44 expression in NEAT1-knockout cells. NEAT1v1 expression in HCC tissues was correlated with poor prognosis and CD44 expression. These results suggest that NEAT1v1 is required for CD44 expression. To our surprise, NEAT1v1 also restored the CSC properties even in CD44-deficient cells, suggesting that NEAT1v1 maintains the properties of CSCs in a CD44-independent manner.

摘要

CD44 是癌症干细胞 (CSC) 的标志物，对于维持肝癌 (HCC) 中的 CSC 特性是必需的。核富集丰富转录本 1 (NEAT1) 是一种长链非编码 RNA (lncRNA)，是 HCC 中的致癌驱动因子。在本研究中，我们研究了基因在与人 HCC 细胞系的肝 CSCs 中与 CD44 表达相关联的意义。通过球体培养、CSC 标志物表达和对抗癌药物的敏感性来评估 CSC 特性。与单层培养相比，球体培养中明显增加了 NEAT1 变体 1 (NEAT1v1) 和变体 2 (NEAT1v2) 以及 CD44 的表达。Neat1v1 的过表达增强了 CSC 特性，而 NEAT1 基因的敲除则抑制了它们，而 Neat1v2 的过表达则没有。Neat1v1 的过表达增加了 CD44 的表达，并通过 NEAT1 敲除消除了这种表达。NEAT1v1 的过表达在 NEAT1 敲除细胞中恢复了 CSC 特性和 CD44 表达。HCC 组织中 NEAT1v1 的表达与预后不良和 CD44 表达相关。这些结果表明，NEAT1v1 是 CD44 表达所必需的。令我们惊讶的是，即使在 CD44 缺陷细胞中，NEAT1v1 也能恢复 CSC 特性，这表明 NEAT1v1 以 CD44 独立的方式维持 CSCs 的特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/e694fba01ae1/ijms-21-01927-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/3b3e4476196a/ijms-21-01927-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/b65798764736/ijms-21-01927-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/2c326a435da0/ijms-21-01927-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/7dccf7a2d729/ijms-21-01927-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/770dfcff3822/ijms-21-01927-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/2aff6cfe875d/ijms-21-01927-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/e694fba01ae1/ijms-21-01927-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/3b3e4476196a/ijms-21-01927-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/b65798764736/ijms-21-01927-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/2c326a435da0/ijms-21-01927-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/7dccf7a2d729/ijms-21-01927-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/770dfcff3822/ijms-21-01927-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/2aff6cfe875d/ijms-21-01927-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a73/7139689/e694fba01ae1/ijms-21-01927-g007.jpg

相似文献

1
NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44.NEAT1 对于肝癌干细胞标志物 CD44 的表达是必需的。
Int J Mol Sci. 2020 Mar 11;21(6):1927. doi: 10.3390/ijms21061927.
2
Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing Autophagy through GABARAP.通过 GABARAP 诱导自噬赋予肝癌细胞放射抵抗性。
Int J Mol Sci. 2022 Jan 10;23(2):711. doi: 10.3390/ijms23020711.
3
CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line.CD44 标准异构体参与维持肝癌细胞系中的癌症干细胞。
Cancer Med. 2019 Feb;8(2):773-782. doi: 10.1002/cam4.1968. Epub 2019 Jan 12.
4
Long non-coding RNA NEAT1 promotes hepatocellular carcinoma cell proliferation through the regulation of miR-129-5p-VCP-IκB.长链非编码RNA NEAT1通过调控miR-129-5p-VCP-IκB促进肝癌细胞增殖。
Am J Physiol Gastrointest Liver Physiol. 2017 Aug 1;313(2):G150-G156. doi: 10.1152/ajpgi.00426.2016. Epub 2017 May 19.
5
The long noncoding RNA NEAT1 contributes to hepatocellular carcinoma development by sponging miR-485 and enhancing the expression of the STAT3.长链非编码 RNA NEAT1 通过海绵吸附 miR-485 并增强 STAT3 的表达促进肝细胞癌的发展。
J Cell Physiol. 2018 Sep;233(9):6733-6741. doi: 10.1002/jcp.26371. Epub 2018 Apr 16.
6
Tumor-associated macrophages produce interleukin 6 and signal via STAT3 to promote expansion of human hepatocellular carcinoma stem cells.肿瘤相关巨噬细胞产生白细胞介素6并通过信号转导和转录激活因子3（STAT3）发出信号，以促进人肝癌干细胞的扩增。
Gastroenterology. 2014 Dec;147(6):1393-404. doi: 10.1053/j.gastro.2014.08.039. Epub 2014 Aug 30.
7
Prognostic relevance of miR-137 in patients with hepatocellular carcinoma.miR-137 在肝细胞癌患者中的预后相关性。
Liver Int. 2017 Feb;37(2):271-279. doi: 10.1111/liv.13213. Epub 2016 Aug 16.
8
ICAM-1-Related Noncoding RNA in Cancer Stem Cells Maintains ICAM-1 Expression in Hepatocellular Carcinoma.肿瘤干细胞中与 ICAM-1 相关的非编码 RNA 维持肝癌中 ICAM-1 的表达。
Clin Cancer Res. 2016 Apr 15;22(8):2041-50. doi: 10.1158/1078-0432.CCR-14-3106. Epub 2015 Dec 14.
9
Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.硫酸乙酰肝素蛋白聚糖-1 及外源性肝素在肝癌球形成中的作用。
Biochem Cell Biol. 2020 Apr;98(2):112-119. doi: 10.1139/bcb-2018-0246. Epub 2019 May 1.
10
NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing PINK1/Parkin-Mediated Mitophagy.NEAT1 通过诱导 PINK1/Parkin 介导的线粒体自噬赋予肝癌细胞放射抗性。
Int J Mol Sci. 2022 Nov 19;23(22):14397. doi: 10.3390/ijms232214397.

引用本文的文献

1
Immune evasion from macrophages by NEAT1-induced CD24 in liver cancer.肝癌中NEAT1诱导的CD24介导的巨噬细胞免疫逃逸
Oncogene. 2025 Aug 12. doi: 10.1038/s41388-025-03537-3.
2
Rewriting the MASLD-associated hepatocellular carcinoma script: Targeting epigenetics and metabolism.改写与代谢相关脂肪性肝病相关的肝细胞癌脚本：靶向表观遗传学和代谢。
Int J Cancer. 2025 Nov 15;157(10):1991-2003. doi: 10.1002/ijc.70047. Epub 2025 Jul 22.
3
The cancer-associated fibroblast facilitates YAP liquid-liquid phase separation to promote cancer cell stemness in HCC.

本文引用的文献

1
Sphere-forming culture enriches liver cancer stem cells and reveals Stearoyl-CoA desaturase 1 as a potential therapeutic target.球体形成培养富集肝癌干细胞并揭示硬脂酰辅酶 A 去饱和酶 1 作为潜在的治疗靶点。
BMC Cancer. 2019 Aug 1;19(1):760. doi: 10.1186/s12885-019-5963-z.
2
Prognostic Effect of Long Noncoding RNA NEAT1 Expression Depends on p53 Mutation Status in Cancer.长链非编码RNA NEAT1表达的预后效应取决于癌症中的p53突变状态。
J Oncol. 2019 May 2;2019:4368068. doi: 10.1155/2019/4368068. eCollection 2019.
3
CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line.
癌症相关成纤维细胞促进YAP液-液相分离，以促进肝癌中的癌细胞干性。
Cell Commun Signal. 2025 May 24;23(1):238. doi: 10.1186/s12964-025-02256-2.
4
Identification and characterization of stromal-like cells with CD207 CD1a phenotype derived from histiocytic lesions - a perspective in vitro model for drug testing.从组织细胞病变中鉴定和表征具有 CD207 CD1a 表型的基质样细胞 - 一种体外药物测试的模型。
BMC Cancer. 2024 Feb 12;24(1):105. doi: 10.1186/s12885-023-11807-0.
5
Recent advances of novel targeted drug delivery systems based on natural medicine monomers against hepatocellular carcinoma.基于天然药物单体的新型靶向给药系统抗肝细胞癌的研究进展
Heliyon. 2024 Jan 18;10(2):e24667. doi: 10.1016/j.heliyon.2024.e24667. eCollection 2024 Jan 30.
6
LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A.长链非编码 RNA NEAT1 通过依赖 KIF11 的 CDKN2A 抑制抑制肝癌细胞衰老。
Clin Transl Med. 2023 Sep;13(9):e1418. doi: 10.1002/ctm2.1418.
7
NEAT1-SOD2 Axis Confers Sorafenib and Lenvatinib Resistance by Activating AKT in Liver Cancer Cell Lines.NEAT1-SOD2轴通过激活肝癌细胞系中的AKT赋予索拉非尼和乐伐替尼耐药性。
Curr Issues Mol Biol. 2023 Jan 29;45(2):1073-1085. doi: 10.3390/cimb45020071.
8
NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing PINK1/Parkin-Mediated Mitophagy.NEAT1 通过诱导 PINK1/Parkin 介导的线粒体自噬赋予肝癌细胞放射抗性。
Int J Mol Sci. 2022 Nov 19;23(22):14397. doi: 10.3390/ijms232214397.
9
Subpopulation cooperation renders drug resistance of hepatobiliary tumor organoids.亚群合作导致肝胆肿瘤类器官产生耐药性。
Chin J Cancer Res. 2022 Aug 30;34(4):422-424. doi: 10.21147/j.issn.1000-9604.2022.04.10.
10
CD44 potentiates hepatocellular carcinoma migration and extrahepatic metastases via the AKT/ERK signaling CXCR4 axis.CD44通过AKT/ERK信号传导CXCR4轴增强肝细胞癌的迁移和肝外转移。
Ann Transl Med. 2022 Jun;10(12):689. doi: 10.21037/atm-22-2482.
CD44 标准异构体参与维持肝癌细胞系中的癌症干细胞。
Cancer Med. 2019 Feb;8(2):773-782. doi: 10.1002/cam4.1968. Epub 2019 Jan 12.
4
CD44s is a crucial ATG7 downstream regulator for stem-like property, invasion, and lung metastasis of human bladder cancer (BC) cells.CD44s 是关键的 ATG7 下游调控因子，可调节人膀胱癌（BC）细胞的干细胞样特性、侵袭和肺转移。
Oncogene. 2019 Apr;38(17):3301-3315. doi: 10.1038/s41388-018-0664-7. Epub 2019 Jan 11.
5
Targeting Cancer Stemness in the Clinic: From Hype to Hope.靶向肿瘤干细胞：从炒作到希望。
Cell Stem Cell. 2019 Jan 3;24(1):25-40. doi: 10.1016/j.stem.2018.11.017. Epub 2018 Dec 27.
6
Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR-101-3p/WEE1 axis.长链非编码 RNA NEAT1_2 的下调通过调节 miR-101-3p/WEE1 轴增强肝癌细胞的放射敏感性。
Cell Biol Int. 2019 Jan;43(1):44-55. doi: 10.1002/cbin.11077.
7
LncRNA NEAT1 promotes the tumorigenesis of colorectal cancer by sponging miR-193a-3p.长链非编码 RNA NEAT1 通过海绵吸附 miR-193a-3p 促进结直肠癌细胞的肿瘤发生。
Cell Prolif. 2019 Jan;52(1):e12526. doi: 10.1111/cpr.12526. Epub 2018 Nov 8.
8
PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.PTBP3 剪接因子通过破坏 NEAT1 和 pre-miR-612 的剪接平衡促进肝癌的发生。
Oncogene. 2018 Dec;37(50):6399-6413. doi: 10.1038/s41388-018-0416-8. Epub 2018 Aug 1.
9
Long non-coding RNA NEAT1-modulated abnormal lipolysis via ATGL drives hepatocellular carcinoma proliferation.长链非编码 RNA NEAT1 通过脂肪甘油三酯酶调控异常脂肪分解促进肝癌增殖。
Mol Cancer. 2018 May 15;17(1):90. doi: 10.1186/s12943-018-0838-5.
10
Cancer stem cells as key drivers of tumour progression.肿瘤干细胞作为肿瘤进展的关键驱动因素。
J Biomed Sci. 2018 Mar 6;25(1):20. doi: 10.1186/s12929-018-0426-4.