Pinto Ana, Barbosa Joana, Albano Helena, Isidro Joana, Teixeira Paula
Escola Superior de Biotecnologia, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health, 1649-016 Lisbon, Portugal.
Microorganisms. 2020 Mar 11;8(3):393. doi: 10.3390/microorganisms8030393.
Probiotics are living microorganisms used as nutritional additives that confer health benefits on the host. Their use in food products is very attractive, especially if they could also inhibit important foodborne pathogens. In this study, antimicrobial activity against several foodborne pathogens was screened for 280 lactic acid bacteria (LAB) isolated from different food products and the probiotic characteristics of bacteriocinogenic isolates were evaluated. Seven out of 280 LAB isolates were selected due to their bacteriocinogenic properties and identified by 16S rRNA gene sequence analysis as ( = 6) and ( = 1). Virulence factors and antibiotic resistances were not detected for any of the isolates. Except for R23, all the isolates were able to survive through the simulated gastrointestinal tract conditions. Only CFF4 was able to adhere to Caco-2 cells after the simulated gastrointestinal tract passage. In conclusion, even though in vivo studies should be performed, CFF4, which was also able to inhibit the growth of foodborne pathogens in vitro, seems to be a potential probiotic to be used in the food industry.
益生菌是用作营养添加剂的活微生物，可给宿主带来健康益处。它们在食品中的应用很有吸引力，特别是如果它们还能抑制重要的食源性病原体。在本研究中，对从不同食品中分离出的280株乳酸菌（LAB）进行了针对几种食源性病原体的抗菌活性筛选，并评估了产细菌素分离株的益生菌特性。280株LAB分离株中有7株因其产细菌素特性而被选，通过16S rRNA基因序列分析鉴定为 （ = 6）和 （ = 1）。未检测到任何分离株的毒力因子和抗生素耐药性。除 R23外，所有分离株都能在模拟胃肠道条件下存活。只有 CFF4在模拟胃肠道通过后能够黏附于Caco-2细胞。总之，尽管应进行体内研究，但在体外也能抑制食源性病原体生长的 CFF4似乎是一种可用于食品工业的潜在益生菌。
