Mandell B F, Spilberg I, Lichtman J
J Immunol. 1977 Apr;118(4):1375-8.
Incubation of human polymorphonuclear leukocytes with colchicine and fluorescein-concanavalin A leads to the formation of a polarized cap of fluorescence not seen if cells are incubated with fluorescein-Con A along. When cells are preincubated with a chemotactic factor before colchicine treatment, the capping is inhibited in a dose-related manner. Studies with alpha-methylmannoside indicate that the caps represent extracellular fluorescein-Con A and are not areas of Con A internalization. Experiments utilizing an irreversible inhibitor of serine esterases suggest that a chemotactic factor-activated enzyme is involved in the inhibition of cap formation in the human neutrophil.
将人多形核白细胞与秋水仙碱和荧光素 - 伴刀豆球蛋白A一起孵育，会导致形成一个极化的荧光帽，而如果细胞仅与荧光素 - 伴刀豆球蛋白A一起孵育则不会出现这种情况。当细胞在秋水仙碱处理前用趋化因子进行预孵育时，帽化以剂量相关的方式受到抑制。用α - 甲基甘露糖苷进行的研究表明，这些帽代表细胞外的荧光素 - 伴刀豆球蛋白A，而不是伴刀豆球蛋白A内化的区域。利用丝氨酸酯酶不可逆抑制剂进行的实验表明，一种趋化因子激活的酶参与了人中性粒细胞帽形成的抑制过程。
