Department of Dermatology, Daqing Oilfield General Hospital, No. 9 Zhongkang Road, Saertu District, Daqing, 163000, Heilongjiang, China.
Department of Stomatology, Daqing Oilfield General Hospital, No. 9 Zhongkang Road, Saertu District, Daqing, 163000, Heilongjiang, China.
Biol Res. 2020 Mar 14;53(1):11. doi: 10.1186/s40659-020-00278-3.
Melanoma is one of the major types of skin cancer. The metastatic melanoma is among the most lethal forms of malignant skin tumors. We hereby aimed to characterize a novel microRNA (miR) in the metastatic melanoma model.
First, we evaluated the expression of miR-107 in melanoma cells and tumor tissues. The comparison between primary and metastatic cancer tissues was also accessed. Next, we examined the impact of miR-107 on melanoma cell proliferation, cell cycle, colony formation, apoptotic activity, migration and matrix invasion. A downstream target of miR-107 was also predicted and validated functionally in melanoma cells.
Our findings showed miR-107 was significantly downregulated in melanoma. Its expression was lowest in metastatic form. Over-expression of miR-107 reduced melanoma cell proliferation, migration and invasion. POU3F2 was identified as the downstream target of miR-107. Over-expression of POU3F2 antagonized miR-107-mediated inhibitory effect on melanoma cells.
Our study has reported miR-107 as a novel tumor suppressive factor in the metastatic melanoma model. It has provided new avenue to manage melanoma and improve the survival rate in the advanced stage.
黑色素瘤是皮肤癌的主要类型之一。转移性黑色素瘤是最致命的恶性皮肤肿瘤之一。本研究旨在对转移性黑色素瘤模型中的一种新型 microRNA（miR）进行特征分析。
首先，我们评估了 miR-107 在黑色素瘤细胞和肿瘤组织中的表达。还比较了原发性和转移性癌组织之间的差异。接下来，我们研究了 miR-107 对黑色素瘤细胞增殖、细胞周期、集落形成、凋亡活性、迁移和基质侵袭的影响。miR-107 的下游靶标也在黑色素瘤细胞中进行了预测和功能验证。
我们的研究结果表明 miR-107 在黑色素瘤中显著下调，在转移性黑色素瘤中表达最低。过表达 miR-107 可降低黑色素瘤细胞的增殖、迁移和侵袭。POU3F2 被鉴定为 miR-107 的下游靶标。过表达 POU3F2 拮抗了 miR-107 对黑色素瘤细胞的抑制作用。
本研究报道了 miR-107 作为转移性黑色素瘤模型中的一种新型肿瘤抑制因子。它为管理黑色素瘤提供了新的途径，并提高了晚期患者的生存率。
