Department of Immunology, Iran University of Medical Sciences, Tehran, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
IUBMB Life. 2020 Sep;72(9):2034-2044. doi: 10.1002/iub.2345. Epub 2020 Jul 20.
Melanoma is a serious form of skin cancers begins in the melanocyte. Micro-RNAs are small noncoding RNA with 19 to 25 nucleotides in length involves in the regulation of a wide range of biological processes. MicroRNAs are affected by an aberrant epigenetic alteration in the tumors that may lead to their dysregulation and formation of cancer. Recently, dysregulation of numerous microRNAs has been reported in different types of cancer. The present study focused on the role of miR-143 in carcinogenesis of melanoma cancer. Here, we evaluated the expression level of miR-143 in three melanoma cell lines in comparison with the normal human epidermal melanocyte cell line. Then, miR-143 gene plasmid transfected into the WM115 cell line, for having the lowest expression of miR-143. In addition, the effect of miR-143 transfection on mRNA and protein levels of metastasis-related genes was performed along with MTT assay, wound healing assay, and flow cytometry. The results showed that mRNA and protein expression levels of metastasis-related genes including MMP-9, E-cadherin, Vimentin, and CXCR4 have been reduced following transfection of miR-143. Moreover, the results of the scratch test showed that miR-143 re-expression inhibited cell migration. Also, the role of miR-143 in the induction of apoptosis and inhibition of proliferation by flow cytometry and MTT was confirmed. As a result, the present study showed that miR-143 was involved in metastatic and apoptotic pathways, suggesting that miR-143 acts as a tumor-suppressor microRNA in melanoma cancer.
黑色素瘤是一种严重的皮肤癌，起源于黑色素细胞。微小 RNA 是一种长度为 19 到 25 个核苷酸的小非编码 RNA，参与广泛的生物过程的调节。微小 RNA 受到肿瘤中异常的表观遗传改变的影响，这可能导致它们的失调和癌症的形成。最近，已经在不同类型的癌症中报道了许多微小 RNA 的失调。本研究集中于 miR-143 在黑色素瘤癌症发生中的作用。在这里，我们评估了三种黑色素瘤细胞系与正常人类表皮黑色素细胞系相比 miR-143 的表达水平。然后，将 miR-143 基因质粒转染到 WM115 细胞系中，因为该细胞系中 miR-143 的表达最低。此外，还进行了 miR-143 转染对与转移相关基因的 mRNA 和蛋白水平的影响，以及 MTT 测定、划痕试验和流式细胞术。结果表明，转染 miR-143 后，与转移相关的基因，包括 MMP-9、E-钙黏蛋白、波形蛋白和 CXCR4 的 mRNA 和蛋白表达水平降低。此外，划痕试验的结果表明，miR-143 的重新表达抑制了细胞迁移。另外，通过流式细胞术和 MTT 证实了 miR-143 在诱导细胞凋亡和抑制增殖中的作用。因此，本研究表明 miR-143 参与了转移和凋亡途径，提示 miR-143 在黑色素瘤癌症中作为肿瘤抑制 microRNA 发挥作用。
