EGCG 对变形链球菌生物膜的抑制作用：一种预防龋病的新方法。
Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis.
机构信息
Pondicherry Centre for Biological Science and Educational Trust, Jawahar Nagar, Pondicherry, India; Biological Sciences Department, College of Science, King Faisal University, Hofuf, 31982, Al Hassa, Saudi Arabia.
Department of Food Science and Biotechnology, Sejong University, Seoul, Republic of Korea.
出版信息
Microb Pathog. 2020 Jun;143:104129. doi: 10.1016/j.micpath.2020.104129. Epub 2020 Mar 10.
Dental caries is a common cause for tooth loss and Streptococcus mutans is identified as the etiologic pathogen. This study evaluates the inhibitory potential of Epigallocatechin gallate (EGCG) on S.mutans glucansucrase enzyme and its biofilm. Glucansucrase binding and the inhibitory potential of EGCG was validated using AutoDock tool and enzyme inhibitory assay. Biofilm inhibitory potential was also confirmed using Scanning Electron Microscopic (SEM) analysis in human tooth samples. Molecular docking revealed that EGCG interacted with GLU 515 and TRP 517 amino acids and binds to glucansucrase. SEM analysis revealed inhibition of S.mutans biofilm by various concentrations of EGCG on surfaces of tooth samples. Bioinformatics and biological assays confirmed that EGCG potentially binds to the S. mutans glucansucrase and inhibits its enzymatic activity. Enzymatic inhibition of glucansucrase attenuated biofilm formation potential of S. mutans on tooth surface. Thus, we conclude that EGCG inhibitory potential of S. mutans biofilm on the tooth surface is a novel approach in prevention of dental caries.
龋齿是牙齿缺失的常见原因，而变形链球菌被确定为病原体。本研究评估了表没食子儿茶素没食子酸酯（EGCG）对变形链球菌葡聚糖蔗糖酶及其生物膜的抑制潜能。使用 AutoDock 工具和酶抑制测定法验证了葡聚糖蔗糖酶结合和 EGCG 的抑制潜能。还通过在人牙样本上的扫描电子显微镜（SEM）分析确认了生物膜抑制潜能。分子对接显示 EGCG 与 GLU515 和 TRP517 氨基酸相互作用，并与葡聚糖蔗糖酶结合。SEM 分析显示，EGCG 以各种浓度抑制牙样本表面上变形链球菌生物膜的形成。生物信息学和生物学测定证实，EGCG 可能与变形链球菌的葡聚糖蔗糖酶结合并抑制其酶活性。葡聚糖蔗糖酶的酶抑制作用减弱了变形链球菌在牙齿表面形成生物膜的能力。因此，我们得出结论，EGCG 对牙表面变形链球菌生物膜的抑制潜能是预防龋齿的一种新方法。
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