Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
Institute of Physical Chemistry (IPC), Abbe Center of Photonics (ACP), Friedrich-Schiller-University, Helmholtzweg 4, Jena, Germany.
Biochim Biophys Acta Mol Basis Dis. 2020 Jun 1;1866(6):165763. doi: 10.1016/j.bbadis.2020.165763. Epub 2020 Mar 10.
Excess circulating fatty acids contribute to endothelial dysfunction that subsequently aggravates the metabolic conditions such as fatty liver diseases. However, the exact mechanism of this event is not fully understood, and the investigation on the effect of a direct exposure to fatty acids together with their subsequent fate is of interest. In this work we employed a chemically specific and label-free techniques such as Raman and CARS microscopies, to investigate the process of lipid droplets (LDs) formation in endothelial cells and hepatocytes after exposure to oleic and palmitic acid. We aimed to observe the changes in the composition of LDs associated with metabolism and degradation of lipids. We were able to characterize the diversity in the formation of LDs in endothelium as compared to hepatocytes, as well as the differences in the formation of LDs and degradation manner with respect to the used fatty acid. Thus, for the first time the spectral characteristics of LDs formed in endothelial cells after incubation with oleic and palmitic acid is presented, including the time-dependent changes in their chemical composition.
过量的循环脂肪酸会导致内皮功能障碍，进而加重脂肪肝等代谢疾病。然而，这一事件的确切机制尚不完全清楚，因此研究直接暴露于脂肪酸及其后续命运的影响很有意义。在这项工作中，我们采用了化学特异性和无标记技术，如拉曼和 CARS 显微镜，来研究油酸和棕榈酸暴露后内皮细胞和肝细胞中脂滴 (LD) 的形成过程。我们旨在观察与脂质代谢和降解相关的 LD 组成变化。我们能够描述与肝细胞相比，在内皮细胞中形成 LD 的多样性，以及与使用的脂肪酸有关的 LD 形成和降解方式的差异。因此，我们首次呈现了孵育油酸和棕榈酸后内皮细胞中形成的 LD 的光谱特征，包括其化学成分随时间的变化。
