School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, PR China.
School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, PR China.
Chemosphere. 2020 Jul;251:126372. doi: 10.1016/j.chemosphere.2020.126372. Epub 2020 Mar 5.
Heavy metal pollution is becoming an increasingly serious problem globally, and cadmium pollution ranks first in the world. Reproduction in insects is affected by cadmium stress in a dose-dependent manner. However, no previous studies have examined the molecular mechanisms underlying the influence of cadmium exposure on insect reproduction. In this study, RNA-Seq was used to investigate changes in ovary gene expression in newly emerged female beet army worms. The beet armyworms were reared under 4 cadmium concentrations: 0 mg/kg (control), low 0.2 mg/kg (L), medium 12.8 mg/kg (M) and high 51.2 mg/kg (H). Compared with the control (CK), a total of 3453 differentially expressed genes (DEGs) were identified in L cadmium stress, including 1791 up-regulated and 1662 down-regulated candidates; in L versus M groups, 982 up-regulated and 658 down-regulated DEGs; and in M versus H groups, 6508 up-regulated and 2000 down-regulated DEGs were identified and the expression patterns of ten genes were verified by q PCR. Many of the identified DEGs were relevant to juvenile hormone and molting hormone biosynthesis, insulin secretion, estrogen signaling, amino acid metabolism and lipid biosynthesis. These data will provide a molecular prospective to understand the ecological risk of heavy metal pollution and are a resource for selecting key genes as targets in gene-editing/silencing technologies for sustainable pest management.
重金属污染正成为全球性的日益严重问题，而镉污染在全球居于首位。昆虫的繁殖受到镉胁迫的影响，呈现出剂量依赖的方式。然而，以前的研究尚未探讨镉暴露对昆虫繁殖影响的分子机制。在本研究中，使用 RNA-Seq 技术研究了新出现的雌性甜菜夜蛾卵巢基因表达的变化。甜菜夜蛾在 4 种镉浓度下进行培养：0 mg/kg（对照）、低浓度 0.2 mg/kg（L）、中浓度 12.8 mg/kg（M）和高浓度 51.2 mg/kg（H）。与对照（CK）相比，在 L 镉胁迫下共鉴定出 3453 个差异表达基因（DEGs），包括 1791 个上调和 1662 个下调候选基因；在 L 与 M 组之间，鉴定出 982 个上调和 658 个下调的 DEGs；在 M 与 H 组之间，鉴定出 6508 个上调和 2000 个下调的 DEGs，并且通过 qPCR 验证了十个基因的表达模式。许多鉴定出的 DEGs 与保幼激素和蜕皮激素生物合成、胰岛素分泌、雌激素信号转导、氨基酸代谢和脂质生物合成有关。这些数据将为理解重金属污染的生态风险提供分子视角，并为选择关键基因作为基因编辑/沉默技术在可持续害虫管理中的靶标提供资源。
