Aquatic Toxicology Laboratory, Department of Zoology, Aligarh Muslim University, India.
Chemosphere. 2020 Jul;251:126311. doi: 10.1016/j.chemosphere.2020.126311. Epub 2020 Mar 5.
The study provides cumulative data on the status of the two water bodies. The study designed revealed physicochemical properties (temperature, dissolved oxygen, pH, total dissolved solids and conductivity) to be in the desirable range, however, amongst the heavy metals excepting for Cd all were found to be higher than the permissible limits set by WHO and USEPA. It was observed that these elements cast their impact on bioindices (hepatosomatic index, condition factor, spleenosomatic index and kidney somatic index), renal marker enzyme (creatine kinase), hepatic marker enzymes (aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase), histology of immune organs (liver, spleen, head-kidney and thymus) and level of serum immunoglobulin (IgM). Further, expression levels of Metallothionein (MT) and Glutathione peroxidase (GPX) genes in immune-related tissues (liver, spleen, head-kidney, thymus and blood) observed indicates metal pollution and abiotic stresses. These alterations are reliable indicators of the cellular and humoral immune response in Cyprinus carpio.
本研究提供了这两个水体状况的累积数据。研究设计显示理化性质（温度、溶解氧、pH 值、总溶解固体和电导率）处于理想范围，但除镉以外的重金属全部高于世界卫生组织和美国环保署规定的允许限值。研究还发现，这些元素对生物指标（肝体比、条件系数、脾体比和肾体比）、肾脏标记酶（肌酸激酶）、肝脏标记酶（天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶）、免疫器官（肝、脾、头肾和胸腺）的组织学以及血清免疫球蛋白（IgM）水平产生了影响。此外，在免疫相关组织（肝、脾、头肾、胸腺和血液）中观察到金属硫蛋白（MT）和谷胱甘肽过氧化物酶（GPX）基因的表达水平表明存在金属污染和非生物胁迫。这些变化是鲤鱼细胞和体液免疫反应的可靠指标。
