Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), División de Ciencias Ambientales, Camino a la Presa San José 2055, Lomas 4a Sección, CP 78216, San Luis Potosí, Mexico.
Universidad del Mar, Campus Puerto Ángel, San Pedro Pochutla, 70902, Oaxaca, Mexico.
Chemosphere. 2020 Jul;251:126404. doi: 10.1016/j.chemosphere.2020.126404. Epub 2020 Mar 4.
The removal efficiency (RE) and bioaerosol emission of a perlite biofilter treating vapors of toluene (T) and/or ethyl acetate (EA) were assessed, under different operating conditions, during 171 days. Under the first stages of operation, a mixture of EA and T was treated, with equivalent inlet loads (ILs) of each compound (ranging from 26 to 84 g m h), achieving a 100% RE of EA, and a maximum elimination capacity (EC) of T of 58.7 g m h. An inhibition of T removal was noted in presence of EA, as T was treated subsequently to EA, along biofilter depth. A 17 days starvation period induced no global deterioration of performance regarding EA removal, but a 50% lower RE of T. Suspension of one contaminant, with interspersed feeding of only one component of the mixture, caused a permanent drop of the RE of EA (to 87.3%), after a T only feeding of 41 days. Flow cytometry (FC) was applied for quantification of bioaerosols, allowing for differentiation between viable, dead and damaged cells. During the overall biofilter operation, bioaerosol emission was not statistically different from bioaerosol retention. However, the biofilter significantly emitted bioaerosols (mostly viable cells) during start-up and IL increase, whereas a global retention of dead cells was observed during the interspersed feeding of one contaminant. Bioaerosols measured by FC (10 Cells m) were three orders of magnitude greater than with plate counting dishes, indicating that FC does not underestimate bioaerosols as culture dependent techniques.
采用斜体的术语在正文中第一次出现时会有相应的中文翻译。
该生物过滤系统使用珍珠岩来处理甲苯（T）和/或乙酸乙酯（EA）蒸气，研究人员评估了在不同操作条件下，该系统在 171 天内的去除效率（RE）和生物气溶胶排放情况。在运行的最初阶段，混合了 EA 和 T 的蒸气被处理，两种化合物的入口负荷（IL）相等（范围从 26 到 84 g m h），实现了 EA 的 100%去除率，T 的最大消除容量（EC）为 58.7 g m h。当 EA 随后被处理，穿过生物过滤器的深度时，T 的去除受到了抑制。在存在 EA 的情况下，17 天的饥饿期没有导致 EA 去除性能的全面恶化，但 T 的去除率降低了 50%。在暂停一种污染物处理的同时，间歇性地仅喂饲混合物的一个成分，在仅 T 喂饲 41 天后，导致 EA 的去除率永久下降（至 87.3%）。流式细胞术（FC）用于定量生物气溶胶，能够区分活细胞、死细胞和受损细胞。在整个生物过滤器运行期间，生物气溶胶排放与生物气溶胶保留没有统计学差异。然而，在启动和入口负荷增加期间，生物过滤器显著排放生物气溶胶（主要是活细胞），而在间歇性喂饲一种污染物期间，观察到死细胞的总体保留。FC 测量的生物气溶胶（10 个细胞 m）比平板计数盘高三个数量级，这表明 FC 没有像依赖培养的技术那样低估生物气溶胶。
