培养皿和无纺布上生长的间充质干细胞之间CD90阳性细胞百分比和成骨分化潜能的比较。
Comparison of percentage of CD90-positive cells and osteogenic differentiation potential between mesenchymal stem cells grown on dish and nonwoven fabric.
作者信息
Fu Bo, Fujiwara Masashi, Takagi Mutsumi
机构信息
Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo, 060-8628, Japan.
出版信息
Cytotechnology. 2020 Jun;72(3):433-444. doi: 10.1007/s10616-020-00390-y. Epub 2020 Mar 13.
Although nonwoven fabric (NWF) has been reported to be a candidate scaffold for the large-scale expansion of mesenchymal stem cells (MSCs), the quality of cells grown in NWF has not been well clarified. In this report, MSCs grown in an NWF disc for 3 weeks showed higher osteogenic differentiation potential and percentage of CD90 (+) cells than MSCs grown on the bottom surface of dish. The amount of the extracellular matrix (ECM) per unit surface area of fibers was larger than that on the bottom surface of the dish in the first 2 weeks of culture. The osteogenic differentiation potential of MSCs inoculated onto cell-free ECM increased with increasing amount of ECM. The higher percentage of CD90 (+) cells and osteogenic differentiation potential of cells grown in an NWF disc than of cells grown on a dish might, at least in part, be due to the higher amount of ECM.
尽管有报道称无纺布（NWF）是间充质干细胞（MSCs）大规模扩增的候选支架，但在NWF中生长的细胞质量尚未得到充分阐明。在本报告中，在NWF圆盘上培养3周的MSCs比在培养皿底部生长的MSCs表现出更高的成骨分化潜力和CD90（+）细胞百分比。在培养的前2周，纤维单位表面积的细胞外基质（ECM）量大于培养皿底部的量。接种到无细胞ECM上的MSCs的成骨分化潜力随ECM量的增加而增加。在NWF圆盘中生长的细胞比在培养皿中生长的细胞具有更高百分比的CD90（+）细胞和成骨分化潜力，这可能至少部分归因于更高的ECM量。
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