Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Inflammation. 2020 Jun;43(3):962-974. doi: 10.1007/s10753-020-01182-4.
Esculetin, a coumarin derivative from various natural plants, has an anti-inflammatory property. In the present study, we examined if esculetin has any salutary effects against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Acute lung injury (ALI) was induced via the intratracheal administration of LPS, and esculetin (20 and 40 mg/kg) was given intraperitoneally 30 min before LPS challenge. After 6 h of LPS administration, lung tissues were collected for analysis. Pretreatment with esculetin significantly attenuated histopathological changes, inflammatory cell infiltration, and production of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, in the lung tissue. Furthermore, esculetin inhibited the protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and nuclear factor-kappa B (NF-κB) pathways and downregulated the expression of RORγt and IL-17 in LPS-induced ALI. Our results indicated that esculetin possesses anti-inflammatory and protective effects against LPS-induced ALI via inhibition of the AKT/ERK/NF-κB and RORγt/IL-17 pathways.
七叶灵是一种从各种天然植物中提取的香豆素衍生物，具有抗炎作用。在本研究中，我们研究了七叶灵是否对 LPS 诱导的急性肺损伤（ALI）有治疗作用。通过气管内给予 LPS 诱导急性肺损伤（ALI），在 LPS 攻击前 30 分钟通过腹腔内给予七叶灵（20 和 40mg/kg）。给予 LPS 6 小时后，收集肺组织进行分析。七叶灵预处理显著减轻了肺组织的组织病理学变化、炎症细胞浸润和促炎细胞因子（如肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6）的产生。此外，七叶灵抑制了蛋白激酶 B（AKT）、细胞外信号调节激酶（ERK）和核因子-κB（NF-κB）途径，并下调了 LPS 诱导的 ALI 中 RORγt 和 IL-17 的表达。我们的结果表明，七叶灵通过抑制 AKT/ERK/NF-κB 和 RORγt/IL-17 途径，对 LPS 诱导的 ALI 具有抗炎和保护作用。
