酶联免疫吸附试验检测猪血清或黏膜型特异性 IgG 和 IgA 全病毒抗体对甲型流感病毒。
Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype-Specific IgG and IgA Whole-Virus Antibody to Influenza A Virus in Swine.
机构信息
Veterinary Diagnostic Laboratory, Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA.
出版信息
Methods Mol Biol. 2020;2123:311-320. doi: 10.1007/978-1-0716-0346-8_22.
Enzyme-linked immunosorbent assays can be used to detect isotype-specific anti-influenza antibodies in biological samples to characterize the porcine immune response to influenza A virus (IAV). The isotype antibody assay is based on an indirect ELISA using whole influenza virus as antigen and commercial antibodies directed against porcine IgG and IgA. Samples such as serum, nasal wash, and bronchoalveolar lavage fluid allow for evaluation of systemic, upper, and lower respiratory tract mucosal antibody responses, respectively. The isotype ELISA assay is performed in a 96-well format using IAV test antigen and anti-swine IgG or IgA detection antibodies conjugated to an enzyme that catalyze a color change reaction. The optical density of the sample is measured using an automated plate reader. The assay is useful to characterize the IgG or IgA immune response to challenge or vaccination against specific IAV isolates in different compartments of the immune system.
酶联免疫吸附测定法可用于检测生物样本中的针对流感病毒的特异性抗体，以表征猪对甲型流感病毒（IAV）的免疫反应。该抗体检测法基于间接酶联免疫吸附测定法，使用整个流感病毒作为抗原，以及针对猪 IgG 和 IgA 的商业抗体。血清、鼻洗液和支气管肺泡灌洗液等样本可分别评估系统、上呼吸道和下呼吸道黏膜的抗体反应。该检测法在 96 孔板中进行，使用 IAV 测试抗原和连接酶的抗猪 IgG 或 IgA 检测抗体，酶可催化颜色变化反应。使用自动平板读数器测量样品的光密度。该检测法可用于描述针对特定 IAV 分离株的免疫挑战或免疫接种后在免疫系统不同部位的 IgG 或 IgA 免疫反应。
Zotero 插件