无标记高通量光声断层成像术在体内疑似循环黑色素瘤肿瘤细胞患者中的应用。
Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo.
机构信息
Washington University in St. Louis, Department of Biomedical Engineering, St. Louis, Missouri, United States.
California Institute of Technology, Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Depa, United States.
出版信息
J Biomed Opt. 2020 Mar;25(3):1-17. doi: 10.1117/1.JBO.25.3.036002.
SIGNIFICANCE
Detection and characterization of circulating tumor cells (CTCs), a key determinant of metastasis, are critical for determining risk of disease progression, understanding metastatic pathways, and facilitating early clinical intervention.
AIM
We aim to demonstrate label-free imaging of suspected melanoma CTCs.
APPROACH
We use a linear-array-based photoacoustic tomography system (LA-PAT) to detect melanoma CTCs, quantify their contrast-to-noise ratios (CNRs), and measure their flow velocities in most of the superficial veins in humans.
RESULTS
With LA-PAT, we successfully imaged suspected melanoma CTCs in patients in vivo, with a CNR >9. CTCs were detected in 3 of 16 patients with stage III or IV melanoma. Among the three CTC-positive patients, two had disease progression; among the 13 CTC-negative patients, 4 showed disease progression.
CONCLUSIONS
We suggest that LA-PAT can detect suspected melanoma CTCs in patients in vivo and has potential clinical applications for disease monitoring in melanoma.
意义
循环肿瘤细胞 (CTC) 的检测和特征分析是转移的关键决定因素，对于确定疾病进展的风险、了解转移途径以及促进早期临床干预至关重要。
目的
我们旨在展示疑似黑色素瘤 CTC 的无标记成像。
方法
我们使用基于线阵的光声层析成像系统 (LA-PAT) 来检测黑色素瘤 CTC，定量其对比噪声比 (CNR)，并测量它们在大多数人体浅表静脉中的血流速度。
结果
通过 LA-PAT，我们成功地对体内疑似黑色素瘤 CTC 进行了成像，其 CNR 大于 9。在 3 期或 4 期黑色素瘤的 16 名患者中检测到了 CTC。在 3 名 CTC 阳性患者中，有 2 人出现疾病进展；在 13 名 CTC 阴性患者中，有 4 人出现疾病进展。
结论
我们建议 LA-PAT 可以在体内检测疑似黑色素瘤 CTC，并有可能在黑色素瘤的疾病监测中得到临床应用。
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